HCF of three numbers

Yield of Protein Calculator

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✖The Volume of top phase is determined by the initial ratio of the two phases and the conditions of the extraction process.ⓘ The Volume of top phase [V_{top phase}]			+10% -10%
✖The optical density of top phase is the optical density of top phase extracts in aqueous two phase extraction.ⓘ The optical density of top phase [O.D_{top phase}]			+10% -10%
✖The Volume of bottom phase is the volume of liquid collected in the bottom phase after two phase extraction.ⓘ The Volume of bottom phase [V_{bottom phase}]			+10% -10%
✖The optical density of bottom phase the optical denisty of the bottom phase extracts in aqeous two phase extraction.ⓘ The optical density of bottom phase [OD_{bottom phase}]			+10% -10%

✖The yield of protein is the amount of protein extracted by aqueous two phase extraction.ⓘ Yield of Protein [Y_{yield of protein}]

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Yield of Protein Solution

STEP 0: Pre-Calculation Summary

Formula Used

The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase)
Y_{yield of protein} = (V_{top phase}*O.D_{top phase})/(V_{bottom phase}*OD_{bottom phase})
This formula uses 5 Variables

Variables Used

The yield of protein - The yield of protein is the amount of protein extracted by aqueous two phase extraction.
The Volume of top phase - (Measured in Cubic Meter) - The Volume of top phase is determined by the initial ratio of the two phases and the conditions of the extraction process.
The optical density of top phase - (Measured in Cubic Meter) - The optical density of top phase is the optical density of top phase extracts in aqueous two phase extraction.
The Volume of bottom phase - (Measured in Cubic Meter) - The Volume of bottom phase is the volume of liquid collected in the bottom phase after two phase extraction.
The optical density of bottom phase - (Measured in Cubic Meter) - The optical density of bottom phase the optical denisty of the bottom phase extracts in aqeous two phase extraction.

STEP 1: Convert Input(s) to Base Unit

The Volume of top phase: 25 Milliliter --> 2.5E-05 Cubic Meter (Check conversion here)
The optical density of top phase: 25 Milliliter --> 2.5E-05 Cubic Meter (Check conversion here)
The Volume of bottom phase: 20 Milliliter --> 2E-05 Cubic Meter (Check conversion here)
The optical density of bottom phase: 20 Milliliter --> 2E-05 Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

Y_{yield of protein} = (V_{top phase}*O.D_{top phase})/(V_{bottom phase}*OD_{bottom phase}) --> (2.5E-05*2.5E-05)/(2E-05*2E-05)

Evaluating ... ...

Y_{yield of protein} = 1.5625

STEP 3: Convert Result to Output's Unit

1.5625 --> No Conversion Required

FINAL ANSWER

1.5625 <-- The yield of protein

(Calculation completed in 00.004 seconds)

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Created by harykrishnan

SRM Institute of Science and Technology (SRMIST), Chennai

harykrishnan has created this Calculator and 10+ more calculators!

Verified by Soupayan banerjee

National University of Judicial Science (NUJS), Kolkata

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Yield of Protein Formula

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What is Aqueous Two Phase Extraction ?

Aqueous two-phase extraction is a liquid-liquid separation technique that uses two immiscible aqueous solutions to partition and separate biomolecules or other substances based on their affinity for the different phases.

What is Protein Yield ?

Protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases.

How to Calculate Yield of Protein?

Yield of Protein calculator uses The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase) to calculate the The yield of protein, The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases. The yield of protein is denoted by Y_{yield of protein} symbol.

How to calculate Yield of Protein using this online calculator? To use this online calculator for Yield of Protein, enter The Volume of top phase (V_{top phase}), The optical density of top phase (O.D_{top phase}), The Volume of bottom phase (V_{bottom phase}) & The optical density of bottom phase (OD_{bottom phase}) and hit the calculate button. Here is how the Yield of Protein calculation can be explained with given input values -> 1.25 = (2.5E-05*2.5E-05)/(2E-05*2E-05).

FAQ

What is Yield of Protein?

The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases and is represented as Y_{yield of protein} = (V_{top phase}*O.D_{top phase})/(V_{bottom phase}*OD_{bottom phase}) or The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase). The Volume of top phase is determined by the initial ratio of the two phases and the conditions of the extraction process, The optical density of top phase is the optical density of top phase extracts in aqueous two phase extraction, The Volume of bottom phase is the volume of liquid collected in the bottom phase after two phase extraction & The optical density of bottom phase the optical denisty of the bottom phase extracts in aqeous two phase extraction.

How to calculate Yield of Protein?

The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases is calculated using The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase). To calculate Yield of Protein, you need The Volume of top phase (V_{top phase}), The optical density of top phase (O.D_{top phase}), The Volume of bottom phase (V_{bottom phase}) & The optical density of bottom phase (OD_{bottom phase}). With our tool, you need to enter the respective value for The Volume of top phase, The optical density of top phase, The Volume of bottom phase & The optical density of bottom phase and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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