Volume of Mobile Phase given Capacity Factor and Partition Coefficient Calculator

✖The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.ⓘ Partition Coefficient [K]			+10% -10%
✖The Volume of Stationary Phase is the amount of motionless part of the chromatography column.ⓘ Volume of Stationary Phase [V_s]			+10% -10%
✖The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.ⓘ Capacity Factor [k^']			+10% -10%

✖The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column.ⓘ Volume of Mobile Phase given Capacity Factor and Partition Coefficient [V_{mobile phase}]

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Volume of Mobile Phase given Capacity Factor and Partition Coefficient Solution

STEP 0: Pre-Calculation Summary

Formula Used

Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor)
V_{mobile phase} = K*(V_s/k^')
This formula uses 4 Variables

Variables Used

Volume of Mobile Phase - (Measured in Cubic Meter) - The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column.
Partition Coefficient - The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.
Volume of Stationary Phase - (Measured in Cubic Meter) - The Volume of Stationary Phase is the amount of motionless part of the chromatography column.
Capacity Factor - The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.

STEP 1: Convert Input(s) to Base Unit

Partition Coefficient: 40 --> No Conversion Required
Volume of Stationary Phase: 7 Liter --> 0.007 Cubic Meter (Check conversion here)
Capacity Factor: 3 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V_{mobile phase} = K*(V_s/k^') --> 40*(0.007/3)

Evaluating ... ...

V_{mobile phase} = 0.0933333333333333

STEP 3: Convert Result to Output's Unit

0.0933333333333333 Cubic Meter -->93.3333333333333 Liter (Check conversion here)

FINAL ANSWER

93.3333333333333 ≈ 93.33333 Liter <-- Volume of Mobile Phase

(Calculation completed in 00.004 seconds)

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< Volume and Concentration of Mobile and Stationary Phase Calculators

Volume of Mobile Phase given Capacity Factor

LaTeX Go Volume of Mobile Phase = (Volume of Stationary Phase*Concentration of Stationary Phase)/(Capacity Factor*Concentration of Mobile Phase)

Volume of Stationary Phase given Capacity Factor

LaTeX Go Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase

Volume of Stationary Phase given Capacity Factor and Partition Coefficient

LaTeX Go Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient

Volume of Mobile Phase given Capacity Factor and Partition Coefficient

LaTeX Go Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor)

Volume of Mobile Phase given Capacity Factor and Partition Coefficient Formula

LaTeX Go

Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor)
V_{mobile phase} = K*(V_s/k^')

What is Chromatography?

A separation process based on the various partitioning coefficients of different solutes between the two phases.
Involving the interaction of solute(s) and two phases
Mobile phase: A gas or liquid that moves through the column.
Stationary phase: A solid or liquid that remains in place.

What are the types of Chromatography?

1) Adsorption chromatography
2) Ion-exchange chromatography
3) Partition chromatography
4) Molecular Size exclusion chromatography
5) Affinity chromatography

How to Calculate Volume of Mobile Phase given Capacity Factor and Partition Coefficient?

Volume of Mobile Phase given Capacity Factor and Partition Coefficient calculator uses Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor) to calculate the Volume of Mobile Phase, The Volume of mobile phase given capacity factor and partition coefficient formula is defined as the product of partition coefficient of the solute to the volume of stationary phase per capacity factor. Volume of Mobile Phase is denoted by V_{mobile phase} symbol.

How to calculate Volume of Mobile Phase given Capacity Factor and Partition Coefficient using this online calculator? To use this online calculator for Volume of Mobile Phase given Capacity Factor and Partition Coefficient, enter Partition Coefficient (K), Volume of Stationary Phase (V_s) & Capacity Factor (k^') and hit the calculate button. Here is how the Volume of Mobile Phase given Capacity Factor and Partition Coefficient calculation can be explained with given input values -> 93333.33 = 40*(0.007/3).

FAQ

What is Volume of Mobile Phase given Capacity Factor and Partition Coefficient?

The Volume of mobile phase given capacity factor and partition coefficient formula is defined as the product of partition coefficient of the solute to the volume of stationary phase per capacity factor and is represented as V_{mobile phase} = K*(V_s/k^') or Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor). The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents, The Volume of Stationary Phase is the amount of motionless part of the chromatography column & The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.

How to calculate Volume of Mobile Phase given Capacity Factor and Partition Coefficient?

The Volume of mobile phase given capacity factor and partition coefficient formula is defined as the product of partition coefficient of the solute to the volume of stationary phase per capacity factor is calculated using Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor). To calculate Volume of Mobile Phase given Capacity Factor and Partition Coefficient, you need Partition Coefficient (K), Volume of Stationary Phase (V_s) & Capacity Factor (k^'). With our tool, you need to enter the respective value for Partition Coefficient, Volume of Stationary Phase & Capacity Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Volume of Mobile Phase?

In this formula, Volume of Mobile Phase uses Partition Coefficient, Volume of Stationary Phase & Capacity Factor. We can use 1 other way(s) to calculate the same, which is/are as follows -

Volume of Mobile Phase = (Volume of Stationary Phase*Concentration of Stationary Phase)/(Capacity Factor*Concentration of Mobile Phase)

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