Thickness of Cell Solution

STEP 0: Pre-Calculation Summary
Formula Used
Thickness of Cell = Absorbance/(Molar Extinction Coefficient*Concentration of Solution)
l = A/(ε*c)
This formula uses 4 Variables
Variables Used
Thickness of Cell - (Measured in Meter) - Thickness of Cell is useful in calculating the concentration of a solution on the basis of its light absorption.
Absorbance - Absorbance is also known as optical density of the solution used in calculating the concentration of a solution on the basis of its light absorption.
Molar Extinction Coefficient - (Measured in Square Meter per Mole) - The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength.
Concentration of Solution - (Measured in Mole per Cubic Meter) - The Concentration of Solution is the quantity of a solute that is contained in a particular quantity of solvent or solution.
STEP 1: Convert Input(s) to Base Unit
Absorbance: 0.92 --> No Conversion Required
Molar Extinction Coefficient: 19 Square Centimeter per Mole --> 0.0019 Square Meter per Mole (Check conversion ​here)
Concentration of Solution: 97 Mole per Cubic Meter --> 97 Mole per Cubic Meter No Conversion Required
STEP 2: Evaluate Formula
Substituting Input Values in Formula
l = A/(ε*c) --> 0.92/(0.0019*97)
Evaluating ... ...
l = 4.99186109603907
STEP 3: Convert Result to Output's Unit
4.99186109603907 Meter -->4991861096.03907 Nanometer (Check conversion ​here)
FINAL ANSWER
4991861096.03907 5E+9 Nanometer <-- Thickness of Cell
(Calculation completed in 00.004 seconds)

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Created by Akshada Kulkarni
National Institute of Information Technology (NIIT), Neemrana
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K J Somaiya College of science (K J Somaiya), Mumbai
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Beer Lambert law Calculators

Concentration of Solution
​ LaTeX ​ Go Concentration of Solution = Absorbance/(Thickness of Cell*Molar Extinction Coefficient)
Beer-Lambert law given Intensity of Radiation
​ LaTeX ​ Go Absorbance = log10(Intensity of Incident Radiation/Intensity of Transmitted Radiation)
Absorbance using Beer-Lambert Law
​ LaTeX ​ Go Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell
Intensity of Incident Radiation
​ LaTeX ​ Go Intensity of Incident Radiation = Intensity of Transmitted Radiation*10^(Absorbance)

Thickness of Cell Formula

​LaTeX ​Go
Thickness of Cell = Absorbance/(Molar Extinction Coefficient*Concentration of Solution)
l = A/(ε*c)

What is Beer- Lambert Law?

Beer-Lambert law is useful in calculating the concentration of a solution on the basis of its light absorption. This law relates the intensity of the transmitted monochromatic light to the concentration of the solution and the thickness of the cell in which the solution is kept.
The molar extinction coefficient of a substance can be determined using a colorimeter or a spectrophotometer as follows. The absorbances of a solution are measured at different
known concentrations using a cell of known thickness (l). The plot of absorbance, A against Concentration of solution, c gives a straight line and its slope is equal to εl.

Define Photochemistry.

In photochemistry, we study the absorption and emission of light by matter. It consists of the study of various photo physical processes and photochemical reactions. Two important photo physical processes are fluorescence and phosphorescence. During fluorescence, light emission takes place in the presence of exciting radiation; but the light emission stops, once the exciting radiation is removed. In contrast to this, during phosphorescence, light emission takes place even after the removal of the exciting radiation.
In photochemical reactions, the substances acquire the necessary activation energy through light absorption. Again this is in contrast to the thermal reactions in which the reactants acquire their activation energy through collisions between molecules.

How to Calculate Thickness of Cell?

Thickness of Cell calculator uses Thickness of Cell = Absorbance/(Molar Extinction Coefficient*Concentration of Solution) to calculate the Thickness of Cell, The Thickness of cell formula is useful in calculating the concentration of a solution on the basis of its light absorption. Thickness of Cell is denoted by l symbol.

How to calculate Thickness of Cell using this online calculator? To use this online calculator for Thickness of Cell, enter Absorbance (A), Molar Extinction Coefficient (ε) & Concentration of Solution (c) and hit the calculate button. Here is how the Thickness of Cell calculation can be explained with given input values -> 5E+18 = 0.92/(0.0019*97).

FAQ

What is Thickness of Cell?
The Thickness of cell formula is useful in calculating the concentration of a solution on the basis of its light absorption and is represented as l = A/(ε*c) or Thickness of Cell = Absorbance/(Molar Extinction Coefficient*Concentration of Solution). Absorbance is also known as optical density of the solution used in calculating the concentration of a solution on the basis of its light absorption, The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength & The Concentration of Solution is the quantity of a solute that is contained in a particular quantity of solvent or solution.
How to calculate Thickness of Cell?
The Thickness of cell formula is useful in calculating the concentration of a solution on the basis of its light absorption is calculated using Thickness of Cell = Absorbance/(Molar Extinction Coefficient*Concentration of Solution). To calculate Thickness of Cell, you need Absorbance (A), Molar Extinction Coefficient (ε) & Concentration of Solution (c). With our tool, you need to enter the respective value for Absorbance, Molar Extinction Coefficient & Concentration of Solution and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Thickness of Cell?
In this formula, Thickness of Cell uses Absorbance, Molar Extinction Coefficient & Concentration of Solution. We can use 2 other way(s) to calculate the same, which is/are as follows -
  • Thickness of Cell = Slope of Line/Molar Extinction Coefficient
  • Thickness of Cell = log10(Intensity of Incident Radiation/Intensity of Transmitted Radiation)*(1/(Molar Extinction Coefficient*Concentration of Solution))
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