Substrate Concentration in Enzymatic Reaction Mechanism Calculator

✖The Reverse Rate Constant is defined as the rate constant for the backward reaction.ⓘ Reverse Rate Constant [k_r]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.ⓘ Forward Rate Constant [k_f]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration in Enzymatic Reaction Mechanism [S]

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Substrate Concentration in Enzymatic Reaction Mechanism Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
S = (k_r*ES)/(k_f*([E₀]-ES))
This formula uses 5 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

STEP 1: Convert Input(s) to Base Unit

Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Forward Rate Constant: 6.9 1 Per Second --> 6.9 1 Per Second No Conversion Required
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (k_r*ES)/(k_f*([E₀]-ES)) --> (20000*10000)/(6.9*(100000-10000))

Evaluating ... ...

S = 322.061191626409

STEP 3: Convert Result to Output's Unit

322.061191626409 Mole per Cubic Meter -->0.322061191626409 Mole per Liter (Check conversion here)

FINAL ANSWER

0.322061191626409 ≈ 0.322061 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.004 seconds)

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< Complex Concentration Calculators

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

LaTeX Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Maximum Rate at Low Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Substrate Concentration in Enzymatic Reaction Mechanism Formula

LaTeX Go

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Substrate Concentration in Enzymatic Reaction Mechanism?

Substrate Concentration in Enzymatic Reaction Mechanism calculator uses Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)) to calculate the Substrate Concentration, The Substrate concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and enzyme-substrate complex. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration in Enzymatic Reaction Mechanism using this online calculator? To use this online calculator for Substrate Concentration in Enzymatic Reaction Mechanism, enter Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Initial Enzyme Concentration ([E₀]) and hit the calculate button. Here is how the Substrate Concentration in Enzymatic Reaction Mechanism calculation can be explained with given input values -> 0.000322 = (20000*10000)/(6.9*(100000-10000)).

FAQ

What is Substrate Concentration in Enzymatic Reaction Mechanism?

The Substrate concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and enzyme-substrate complex and is represented as S = (k_r*ES)/(k_f*([E₀]-ES)) or Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). The Reverse Rate Constant is defined as the rate constant for the backward reaction, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Forward Rate Constant is defined as the rate constant for the forward occurring reaction & The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

How to calculate Substrate Concentration in Enzymatic Reaction Mechanism?

The Substrate concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and enzyme-substrate complex is calculated using Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). To calculate Substrate Concentration in Enzymatic Reaction Mechanism, you need Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Initial Enzyme Concentration ([E₀]). With our tool, you need to enter the respective value for Reverse Rate Constant, Enzyme Substrate Complex Concentration, Forward Rate Constant & Initial Enzyme Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Reverse Rate Constant, Enzyme Substrate Complex Concentration, Forward Rate Constant & Initial Enzyme Concentration. We can use 3 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

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