Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Calculator

✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Enzyme Inhibitor Dissociation Constant [K_i]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration [S]

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Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)
S = (ES*(K_M*(1+(I/K_i))))/(([E₀])-ES)
This formula uses 6 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

STEP 1: Convert Input(s) to Base Unit

Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (ES*(K_M*(1+(I/K_i))))/(([E₀])-ES) --> (10000*(3000*(1+(9000/19000))))/((100000)-10000)

Evaluating ... ...

S = 491.228070175439

STEP 3: Convert Result to Output's Unit

491.228070175439 Mole per Cubic Meter -->0.491228070175439 Mole per Liter (Check conversion here)

FINAL ANSWER

0.491228070175439 ≈ 0.491228 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.007 seconds)

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< Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

LaTeX Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

LaTeX Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

LaTeX Go

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration calculator uses Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration) to calculate the Substrate Concentration, The Substrate concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration using this online calculator? To use this online calculator for Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration, enter Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i) & Initial Enzyme Concentration ([E₀]) and hit the calculate button. Here is how the Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration calculation can be explained with given input values -> 0.000491 = (10000*(3000*(1+(9000/19000))))/((100000)-10000).

FAQ

What is Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

The Substrate concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km and is represented as S = (ES*(K_M*(1+(I/K_i))))/(([E₀])-ES) or Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration). The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system, The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured & The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

How to calculate Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

The Substrate concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km is calculated using Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration). To calculate Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration, you need Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i) & Initial Enzyme Concentration ([E₀]). With our tool, you need to enter the respective value for Enzyme Substrate Complex Concentration, Michaelis Constant, Inhibitor Concentration, Enzyme Inhibitor Dissociation Constant & Initial Enzyme Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Enzyme Substrate Complex Concentration, Michaelis Constant, Inhibitor Concentration, Enzyme Inhibitor Dissociation Constant & Initial Enzyme Concentration. We can use 3 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

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