What is competitive Inhibition?
In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.
How to Calculate Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?
Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration calculator uses Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration) to calculate the Substrate Concentration, The Substrate concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Substrate Concentration is denoted by S symbol.
How to calculate Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration using this online calculator? To use this online calculator for Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration, enter Enzyme Substrate Complex Concentration (ES), Michaelis Constant (KM), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (Ki) & Initial Enzyme Concentration ([E0]) and hit the calculate button. Here is how the Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration calculation can be explained with given input values -> 0.000491 = (10000*(3000*(1+(9000/19000))))/((100000)-10000).