Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Calculator

✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.ⓘ Catalytic Rate Constant [k_cat]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration [S]

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Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
S = (V₀*K_M)/(k_cat*[E₀])
This formula uses 5 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

STEP 1: Convert Input(s) to Base Unit

Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (V₀*K_M)/(k_cat*[E₀]) --> (450*3000)/(0.65*100000)

Evaluating ... ...

S = 20.7692307692308

STEP 3: Convert Result to Output's Unit

20.7692307692308 Mole per Cubic Meter -->0.0207692307692308 Mole per Liter (Check conversion here)

FINAL ANSWER

0.0207692307692308 ≈ 0.020769 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.004 seconds)

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< Complex Concentration Calculators

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

LaTeX Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Maximum Rate at Low Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula

LaTeX Go

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
S = (V₀*K_M)/(k_cat*[E₀])

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration calculator uses Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration) to calculate the Substrate Concentration, The Substrate concentration if Michaelis constant is very large than substrate concentration formula is defined as the relation with initial reaction rate and initial enzyme concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration using this online calculator? To use this online calculator for Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration, enter Initial Reaction Rate (V₀), Michaelis Constant (K_M), Catalytic Rate Constant (k_cat) & Initial Enzyme Concentration ([E₀]) and hit the calculate button. Here is how the Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration calculation can be explained with given input values -> 2.1E-5 = (450*3000)/(0.65*100000).

FAQ

What is Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

The Substrate concentration if Michaelis constant is very large than substrate concentration formula is defined as the relation with initial reaction rate and initial enzyme concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant and is represented as S = (V₀*K_M)/(k_cat*[E₀]) or Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration). The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system, The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product & The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

How to calculate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

The Substrate concentration if Michaelis constant is very large than substrate concentration formula is defined as the relation with initial reaction rate and initial enzyme concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant is calculated using Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration). To calculate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration, you need Initial Reaction Rate (V₀), Michaelis Constant (K_M), Catalytic Rate Constant (k_cat) & Initial Enzyme Concentration ([E₀]). With our tool, you need to enter the respective value for Initial Reaction Rate, Michaelis Constant, Catalytic Rate Constant & Initial Enzyme Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Initial Reaction Rate, Michaelis Constant, Catalytic Rate Constant & Initial Enzyme Concentration. We can use 3 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

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