Substrate Concentration given Modifying Factor in Michaelis Menten Equation Calculator

✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.ⓘ Enzyme Modifying Factor [α]			+10% -10%
✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Enzyme Substrate Modifying Factor [α^']			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration given Modifying Factor in Michaelis Menten Equation [S]

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Substrate Concentration given Modifying Factor in Michaelis Menten Equation Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)
S = (V₀*(α/α^')*K_M)/(((1/α^')*V_max)-V₀)
This formula uses 6 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Enzyme Modifying Factor - The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

STEP 1: Convert Input(s) to Base Unit

Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Enzyme Modifying Factor: 5 --> No Conversion Required
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (V₀*(α/α^')*K_M)/(((1/α^')*V_max)-V₀) --> (450*(5/2)*3000)/(((1/2)*40000)-450)

Evaluating ... ...

S = 172.634271099744

STEP 3: Convert Result to Output's Unit

172.634271099744 Mole per Cubic Meter -->0.172634271099744 Mole per Liter (Check conversion here)

FINAL ANSWER

0.172634271099744 ≈ 0.172634 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.004 seconds)

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< Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

LaTeX Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

LaTeX Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

Substrate Concentration given Modifying Factor in Michaelis Menten Equation Formula

LaTeX Go

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Substrate Concentration given Modifying Factor in Michaelis Menten Equation?

Substrate Concentration given Modifying Factor in Michaelis Menten Equation calculator uses Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate) to calculate the Substrate Concentration, The Substrate concentration given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration given Modifying Factor in Michaelis Menten Equation using this online calculator? To use this online calculator for Substrate Concentration given Modifying Factor in Michaelis Menten Equation, enter Initial Reaction Rate (V₀), Enzyme Modifying Factor (α), Enzyme Substrate Modifying Factor (α^'), Michaelis Constant (K_M) & Maximum Rate (V_max) and hit the calculate button. Here is how the Substrate Concentration given Modifying Factor in Michaelis Menten Equation calculation can be explained with given input values -> 0.000173 = (450*(5/2)*3000)/(((1/2)*40000)-450).

FAQ

What is Substrate Concentration given Modifying Factor in Michaelis Menten Equation?

The Substrate concentration given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation and is represented as S = (V₀*(α/α^')*K_M)/(((1/α^')*V_max)-V₀) or Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate). The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place, The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme, The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system & The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

How to calculate Substrate Concentration given Modifying Factor in Michaelis Menten Equation?

The Substrate concentration given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation is calculated using Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate). To calculate Substrate Concentration given Modifying Factor in Michaelis Menten Equation, you need Initial Reaction Rate (V₀), Enzyme Modifying Factor (α), Enzyme Substrate Modifying Factor (α^'), Michaelis Constant (K_M) & Maximum Rate (V_max). With our tool, you need to enter the respective value for Initial Reaction Rate, Enzyme Modifying Factor, Enzyme Substrate Modifying Factor, Michaelis Constant & Maximum Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Initial Reaction Rate, Enzyme Modifying Factor, Enzyme Substrate Modifying Factor, Michaelis Constant & Maximum Rate. We can use 3 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

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