Substrate Concentration given Maximum Rate at Low Concentration Calculator

✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration given Maximum Rate at Low Concentration [S]

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Substrate Concentration given Maximum Rate at Low Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
S = (V₀*K_M)/V_max
This formula uses 4 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

STEP 1: Convert Input(s) to Base Unit

Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (V₀*K_M)/V_max --> (450*3000)/40000

Evaluating ... ...

S = 33.75

STEP 3: Convert Result to Output's Unit

33.75 Mole per Cubic Meter -->0.03375 Mole per Liter (Check conversion here)

FINAL ANSWER

0.03375 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.004 seconds)

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< Complex Concentration Calculators

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

LaTeX Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Maximum Rate at Low Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Substrate Concentration given Maximum Rate at Low Concentration Formula

LaTeX Go

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
S = (V₀*K_M)/V_max

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Substrate Concentration given Maximum Rate at Low Concentration?

Substrate Concentration given Maximum Rate at Low Concentration calculator uses Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate to calculate the Substrate Concentration, The Substrate concentration given maximum rate at low concentration formula is defined as the relation with an initial rate of the reaction and with a maximum rate of the system. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration given Maximum Rate at Low Concentration using this online calculator? To use this online calculator for Substrate Concentration given Maximum Rate at Low Concentration, enter Initial Reaction Rate (V₀), Michaelis Constant (K_M) & Maximum Rate (V_max) and hit the calculate button. Here is how the Substrate Concentration given Maximum Rate at Low Concentration calculation can be explained with given input values -> 3.4E-5 = (450*3000)/40000.

FAQ

What is Substrate Concentration given Maximum Rate at Low Concentration?

The Substrate concentration given maximum rate at low concentration formula is defined as the relation with an initial rate of the reaction and with a maximum rate of the system. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant and is represented as S = (V₀*K_M)/V_max or Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate. The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system & The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

How to calculate Substrate Concentration given Maximum Rate at Low Concentration?

The Substrate concentration given maximum rate at low concentration formula is defined as the relation with an initial rate of the reaction and with a maximum rate of the system. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant is calculated using Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate. To calculate Substrate Concentration given Maximum Rate at Low Concentration, you need Initial Reaction Rate (V₀), Michaelis Constant (K_M) & Maximum Rate (V_max). With our tool, you need to enter the respective value for Initial Reaction Rate, Michaelis Constant & Maximum Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Initial Reaction Rate, Michaelis Constant & Maximum Rate. We can use 3 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

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