Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants Calculator

✖The Reverse Rate Constant is defined as the rate constant for the backward reaction.ⓘ Reverse Rate Constant [k_r]			+10% -10%
✖The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.ⓘ Catalytic Rate Constant [k_cat]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.ⓘ Forward Rate Constant [k_f]			+10% -10%
✖The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.ⓘ Catalyst Concentration [E]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants [S]

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Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)
S = ((k_r+k_cat)*ES)/(k_f*E)
This formula uses 6 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Catalyst Concentration - (Measured in Mole per Cubic Meter) - The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.

STEP 1: Convert Input(s) to Base Unit

Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion here)
Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Forward Rate Constant: 6.9 1 Per Second --> 6.9 1 Per Second No Conversion Required
Catalyst Concentration: 25 Mole per Liter --> 25000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = ((k_r+k_cat)*ES)/(k_f*E) --> ((20000+0.65)*10000)/(6.9*25000)

Evaluating ... ...

S = 1159.45797101449

STEP 3: Convert Result to Output's Unit

1159.45797101449 Mole per Cubic Meter -->1.15945797101449 Mole per Liter (Check conversion here)

FINAL ANSWER

1.15945797101449 ≈ 1.159458 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.004 seconds)

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< Complex Concentration Calculators

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

LaTeX Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Maximum Rate at Low Concentration

LaTeX Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants Formula

LaTeX Go

Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)
S = ((k_r+k_cat)*ES)/(k_f*E)

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants?

Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants calculator uses Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration) to calculate the Substrate Concentration, The Substrate concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with enzymatic catalyst concentration and with an enzyme-substrate complex concentration in an enzymatic reaction mechanism. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants using this online calculator? To use this online calculator for Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants, enter Reverse Rate Constant (k_r), Catalytic Rate Constant (k_cat), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Catalyst Concentration (E) and hit the calculate button. Here is how the Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants calculation can be explained with given input values -> 0.001159 = ((20000+0.65)*10000)/(6.9*25000).

FAQ

What is Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants?

The Substrate concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with enzymatic catalyst concentration and with an enzyme-substrate complex concentration in an enzymatic reaction mechanism and is represented as S = ((k_r+k_cat)*ES)/(k_f*E) or Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration). The Reverse Rate Constant is defined as the rate constant for the backward reaction, The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Forward Rate Constant is defined as the rate constant for the forward occurring reaction & The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.

How to calculate Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants?

The Substrate concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with enzymatic catalyst concentration and with an enzyme-substrate complex concentration in an enzymatic reaction mechanism is calculated using Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration). To calculate Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants, you need Reverse Rate Constant (k_r), Catalytic Rate Constant (k_cat), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Catalyst Concentration (E). With our tool, you need to enter the respective value for Reverse Rate Constant, Catalytic Rate Constant, Enzyme Substrate Complex Concentration, Forward Rate Constant & Catalyst Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Reverse Rate Constant, Catalytic Rate Constant, Enzyme Substrate Complex Concentration, Forward Rate Constant & Catalyst Concentration. We can use 3 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))

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