Substrate Concentration given Apparent value of Michaelis Menten Constant Calculator

✖The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.ⓘ Apparent Michaelis Constant [Km^app]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration given Apparent value of Michaelis Menten Constant [S]

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Substrate Concentration given Apparent value of Michaelis Menten Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
S = (Km^app*V₀)/(V_max-V₀)
This formula uses 4 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Apparent Michaelis Constant - (Measured in Mole per Cubic Meter) - The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

STEP 1: Convert Input(s) to Base Unit

Apparent Michaelis Constant: 12 Mole per Liter --> 12000 Mole per Cubic Meter (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (Km^app*V₀)/(V_max-V₀) --> (12000*450)/(40000-450)

Evaluating ... ...

S = 136.53603034134

STEP 3: Convert Result to Output's Unit

136.53603034134 Mole per Cubic Meter -->0.13653603034134 Mole per Liter (Check conversion here)

FINAL ANSWER

0.13653603034134 ≈ 0.136536 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.004 seconds)

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< Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

LaTeX Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

LaTeX Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

Substrate Concentration given Apparent value of Michaelis Menten Constant Formula

LaTeX Go

Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
S = (Km^app*V₀)/(V_max-V₀)

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Substrate Concentration given Apparent value of Michaelis Menten Constant?

Substrate Concentration given Apparent value of Michaelis Menten Constant calculator uses Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate) to calculate the Substrate Concentration, The Substrate concentration given apparent value of Michaelis Menten constant formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km(app). Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration given Apparent value of Michaelis Menten Constant using this online calculator? To use this online calculator for Substrate Concentration given Apparent value of Michaelis Menten Constant, enter Apparent Michaelis Constant (Km^app), Initial Reaction Rate (V₀) & Maximum Rate (V_max) and hit the calculate button. Here is how the Substrate Concentration given Apparent value of Michaelis Menten Constant calculation can be explained with given input values -> 0.000137 = (12000*450)/(40000-450).

FAQ

What is Substrate Concentration given Apparent value of Michaelis Menten Constant?

The Substrate concentration given apparent value of Michaelis Menten constant formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km(app) and is represented as S = (Km^app*V₀)/(V_max-V₀) or Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate). The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor, The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place & The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

How to calculate Substrate Concentration given Apparent value of Michaelis Menten Constant?

The Substrate concentration given apparent value of Michaelis Menten constant formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km(app) is calculated using Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate). To calculate Substrate Concentration given Apparent value of Michaelis Menten Constant, you need Apparent Michaelis Constant (Km^app), Initial Reaction Rate (V₀) & Maximum Rate (V_max). With our tool, you need to enter the respective value for Apparent Michaelis Constant, Initial Reaction Rate & Maximum Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Apparent Michaelis Constant, Initial Reaction Rate & Maximum Rate. We can use 3 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

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