Substrate Concentration from Michaelis Menten Kinetics Equation Calculator

✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration from Michaelis Menten Kinetics Equation [S]

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Substrate Concentration from Michaelis Menten Kinetics Equation Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
S = (K_M*V₀)/(V_max-V₀)
This formula uses 4 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

STEP 1: Convert Input(s) to Base Unit

Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (K_M*V₀)/(V_max-V₀) --> (3000*450)/(40000-450)

Evaluating ... ...

S = 34.134007585335

STEP 3: Convert Result to Output's Unit

34.134007585335 Mole per Cubic Meter -->0.034134007585335 Mole per Liter (Check conversion here)

FINAL ANSWER

0.034134007585335 ≈ 0.034134 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.004 seconds)

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< Michaelis Menten Kinetics Equation Calculators

Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate

Maximum Rate of System from Michaelis Menten Kinetics equation

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/Substrate Concentration

Substrate Concentration from Michaelis Menten Kinetics Equation

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Michaelis Constant from Michaelis Menten kinetics equation

LaTeX Go Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)

Substrate Concentration from Michaelis Menten Kinetics Equation Formula

LaTeX Go

Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
S = (K_M*V₀)/(V_max-V₀)

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Substrate Concentration from Michaelis Menten Kinetics Equation?

Substrate Concentration from Michaelis Menten Kinetics Equation calculator uses Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate) to calculate the Substrate Concentration, The Substrate concentration from Michaelis Menten kinetics equation formula is defined as the relation with the initial rate constant and maximum rate achieved by the system. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration from Michaelis Menten Kinetics Equation using this online calculator? To use this online calculator for Substrate Concentration from Michaelis Menten Kinetics Equation, enter Michaelis Constant (K_M), Initial Reaction Rate (V₀) & Maximum Rate (V_max) and hit the calculate button. Here is how the Substrate Concentration from Michaelis Menten Kinetics Equation calculation can be explained with given input values -> 3.4E-5 = (3000*450)/(40000-450).

FAQ

What is Substrate Concentration from Michaelis Menten Kinetics Equation?

The Substrate concentration from Michaelis Menten kinetics equation formula is defined as the relation with the initial rate constant and maximum rate achieved by the system and is represented as S = (K_M*V₀)/(V_max-V₀) or Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate). The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system, The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place & The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

How to calculate Substrate Concentration from Michaelis Menten Kinetics Equation?

The Substrate concentration from Michaelis Menten kinetics equation formula is defined as the relation with the initial rate constant and maximum rate achieved by the system is calculated using Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate). To calculate Substrate Concentration from Michaelis Menten Kinetics Equation, you need Michaelis Constant (K_M), Initial Reaction Rate (V₀) & Maximum Rate (V_max). With our tool, you need to enter the respective value for Michaelis Constant, Initial Reaction Rate & Maximum Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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