Reverse Rate Constant in Enzymatic Reaction Mechanism Calculator

✖The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.ⓘ Forward Rate Constant [k_f]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%

✖The Reverse Rate Constant is defined as the rate constant for the backward reaction.ⓘ Reverse Rate Constant in Enzymatic Reaction Mechanism [k_r]

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Reverse Rate Constant in Enzymatic Reaction Mechanism Solution

STEP 0: Pre-Calculation Summary

Formula Used

Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration
k_r = (k_f*S*([E₀]-ES))/ES
This formula uses 5 Variables

Variables Used

Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

STEP 1: Convert Input(s) to Base Unit

Forward Rate Constant: 6.9 1 Per Second --> 6.9 1 Per Second No Conversion Required
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

k_r = (k_f*S*([E₀]-ES))/ES --> (6.9*1500*(100000-10000))/10000

Evaluating ... ...

k_r = 93150

STEP 3: Convert Result to Output's Unit

93150 Mole per Cubic Meter Second -->93.15 Mole per Liter Second (Check conversion here)

FINAL ANSWER

93.15 Mole per Liter Second <-- Reverse Rate Constant

(Calculation completed in 00.004 seconds)

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< Rate Constants of Enzymatic Reaction Calculators

Forward Rate Constant in Enzymatic Reaction mechanism

LaTeX Go Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))

Reverse Rate Constant in Enzymatic Reaction Mechanism

LaTeX Go Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration

Catalytic Rate Constant at Low Substrate Concentration

LaTeX Go Catalytic Rate Constant = (Initial Reaction Rate*Michaelis Constant)/(Initial Enzyme Concentration*Substrate Concentration)

Dissociation Rate Constant in Enzymatic Reaction Mechanism

LaTeX Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant

Reverse Rate Constant in Enzymatic Reaction Mechanism Formula

LaTeX Go

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Reverse Rate Constant in Enzymatic Reaction Mechanism?

Reverse Rate Constant in Enzymatic Reaction Mechanism calculator uses Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration to calculate the Reverse Rate Constant, The Reverse rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward reaction and with the concentration of enzyme and substrate. Reverse Rate Constant is denoted by k_r symbol.

How to calculate Reverse Rate Constant in Enzymatic Reaction Mechanism using this online calculator? To use this online calculator for Reverse Rate Constant in Enzymatic Reaction Mechanism, enter Forward Rate Constant (k_f), Substrate Concentration (S), Initial Enzyme Concentration ([E₀]) & Enzyme Substrate Complex Concentration (ES) and hit the calculate button. Here is how the Reverse Rate Constant in Enzymatic Reaction Mechanism calculation can be explained with given input values -> 0.09315 = (6.9*1500*(100000-10000))/10000.

FAQ

What is Reverse Rate Constant in Enzymatic Reaction Mechanism?

The Reverse rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward reaction and with the concentration of enzyme and substrate and is represented as k_r = (k_f*S*([E₀]-ES))/ES or Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration. The Forward Rate Constant is defined as the rate constant for the forward occurring reaction, The Substrate Concentration is the number of moles of substrate per liter solution, The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction & The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

How to calculate Reverse Rate Constant in Enzymatic Reaction Mechanism?

The Reverse rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward reaction and with the concentration of enzyme and substrate is calculated using Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration. To calculate Reverse Rate Constant in Enzymatic Reaction Mechanism, you need Forward Rate Constant (k_f), Substrate Concentration (S), Initial Enzyme Concentration ([E₀]) & Enzyme Substrate Complex Concentration (ES). With our tool, you need to enter the respective value for Forward Rate Constant, Substrate Concentration, Initial Enzyme Concentration & Enzyme Substrate Complex Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Reverse Rate Constant?

In this formula, Reverse Rate Constant uses Forward Rate Constant, Substrate Concentration, Initial Enzyme Concentration & Enzyme Substrate Complex Concentration. We can use 3 other way(s) to calculate the same, which is/are as follows -

Reverse Rate Constant = (Dissociation Rate Constant*Forward Rate Constant)
Reverse Rate Constant = ((Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Catalytic Rate Constant
Reverse Rate Constant = (Michaelis Constant*Forward Rate Constant)-Catalytic Rate Constant

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