LCM calculator

Retention Volume using Retention Factor Calculator

Chemistry Engineering Financial Health More >>

↳

Biochemistry Analytical chemistry Atmospheric Chemistry Atomic structure More >>

⤿

Osmolality Bacteriology Genomics Hemodynamics More >>

✖Void Volume is the volume of mobile phase in a column.ⓘ Void Volume [V₀]			+10% -10%
✖Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.ⓘ Retention Factor [R_f]			+10% -10%

✖The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.ⓘ Retention Volume using Retention Factor [V_R]

⎘ Copy

👎

Formula

LaTeX

Reset

👍

Download Chemistry Formula PDF PDF Image

Retention Volume using Retention Factor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Retention Volume = Void Volume*(Retention Factor+1)
V_R = V₀*(R_f+1)
This formula uses 3 Variables

Variables Used

Retention Volume - (Measured in Cubic Meter) - The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.
Void Volume - (Measured in Cubic Meter) - Void Volume is the volume of mobile phase in a column.
Retention Factor - Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.

STEP 1: Convert Input(s) to Base Unit

Void Volume: 5.6 Milliliter --> 5.6E-06 Cubic Meter (Check conversion here)
Retention Factor: 10 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V_R = V₀*(R_f+1) --> 5.6E-06*(10+1)

Evaluating ... ...

V_R = 6.16E-05

STEP 3: Convert Result to Output's Unit

6.16E-05 Cubic Meter -->0.0616 Liter (Check conversion here)

FINAL ANSWER

0.0616 Liter <-- Retention Volume

(Calculation completed in 00.004 seconds)

You are here -

Home » Chemistry » Biochemistry » Osmolality » Retention Volume using Retention Factor

Credits

Created by Soupayan banerjee

National University of Judicial Science (NUJS), Kolkata

Soupayan banerjee has created this Calculator and 200+ more calculators!

Verified by Pratibha

Amity Institute Of Applied Sciences (AIAS, Amity University), Noida, India

Pratibha has verified this Calculator and 50+ more calculators!

< Osmolality Calculators

Increase of Splicing Potential by Wild-type Sequence

LaTeX Go Splicing Potential = log10(Weight Factor for Increase of Splicing Potential)

Decrease of Splicing Potential by Mutant Sequence

LaTeX Go Splicing Potential = -log10(Weight Factor)

Plasma Osmolality

LaTeX Go Plasma Osmolality = (2*Sodium Concentration in Plasma)

Plasma Concentration using Plasma Osmolality

LaTeX Go Sodium Concentration in Plasma = Plasma Osmolality/2

Retention Volume using Retention Factor Formula

LaTeX Go

Retention Volume = Void Volume*(Retention Factor+1)
V_R = V₀*(R_f+1)

What is Column Chromatography?

Column chromatography separates substances based on differential adsorption of compounds to the adsorbent as the compounds move through the column at different rates which allow them to get separated in fractions. This technique can be used on a small scale as well as large scale to purify materials that can be used in future experiments. This method is a type of adsorption chromatography technique.

How to Calculate Retention Volume using Retention Factor?

Retention Volume using Retention Factor calculator uses Retention Volume = Void Volume*(Retention Factor+1) to calculate the Retention Volume, The Retention Volume using Retention Factor formula is defined as the volume that has passed through the column since the target molecule was introduced onto the column. Retention Volume is denoted by V_R symbol.

How to calculate Retention Volume using Retention Factor using this online calculator? To use this online calculator for Retention Volume using Retention Factor, enter Void Volume (V₀) & Retention Factor (R_f) and hit the calculate button. Here is how the Retention Volume using Retention Factor calculation can be explained with given input values -> 61.6 = 5.6E-06*(10+1).

FAQ

What is Retention Volume using Retention Factor?

The Retention Volume using Retention Factor formula is defined as the volume that has passed through the column since the target molecule was introduced onto the column and is represented as V_R = V₀*(R_f+1) or Retention Volume = Void Volume*(Retention Factor+1). Void Volume is the volume of mobile phase in a column & Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.

How to calculate Retention Volume using Retention Factor?

The Retention Volume using Retention Factor formula is defined as the volume that has passed through the column since the target molecule was introduced onto the column is calculated using Retention Volume = Void Volume*(Retention Factor+1). To calculate Retention Volume using Retention Factor, you need Void Volume (V₀) & Retention Factor (R_f). With our tool, you need to enter the respective value for Void Volume & Retention Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Retention Volume?

In this formula, Retention Volume uses Void Volume & Retention Factor. We can use 1 other way(s) to calculate the same, which is/are as follows -

Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16))

Home

FREE PDFs

🔍 Search