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Quenching Concentration Calculator

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Fluoroscence and Phosphorescence Quantum Yield and Singlet Llifetime

✖Initial Intensity flux of radiant energy is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.ⓘ Initial Intensity [Io]			+10% -10%
✖Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.ⓘ Fluorosence Intensity [I_F]			+10% -10%
✖Stern Volmner Constant is the bimolecular rate constant of the fluorescence quenching process due to a short-range interaction of species.ⓘ Stern Volmner Constant [K_SV]			+10% -10%

✖Quencher Concentration is the concentration of substance that decreases fluoroscence intensity.ⓘ Quenching Concentration [[Q1]]

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Quenching Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant
[Q1] = ((Io/I_F)-1)/K_SV
This formula uses 4 Variables

Variables Used

Quencher Concentration - Quencher Concentration is the concentration of substance that decreases fluoroscence intensity.
Initial Intensity - (Measured in Watt per Square Meter) - Initial Intensity flux of radiant energy is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.
Fluorosence Intensity - (Measured in Watt per Square Meter) - Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.
Stern Volmner Constant - Stern Volmner Constant is the bimolecular rate constant of the fluorescence quenching process due to a short-range interaction of species.

STEP 1: Convert Input(s) to Base Unit

Initial Intensity: 500 Watt per Square Meter --> 500 Watt per Square Meter No Conversion Required
Fluorosence Intensity: 240 Watt per Square Meter --> 240 Watt per Square Meter No Conversion Required
Stern Volmner Constant: 0.005 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

[Q1] = ((Io/I_F)-1)/K_SV --> ((500/240)-1)/0.005

Evaluating ... ...

[Q1] = 216.666666666667

STEP 3: Convert Result to Output's Unit

216.666666666667 --> No Conversion Required

FINAL ANSWER

216.666666666667 ≈ 216.6667 <-- Quencher Concentration

(Calculation completed in 00.004 seconds)

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University of Calcutta (CU), Kolkata

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National University of Judicial Science (NUJS), Kolkata

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< Emission Spectroscopy Calculators

Degree of Exciplex Formation

LaTeX Go Degree of Exciplex Formation = (Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex)/(1+(Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex))

Collisional Energy Transfer

LaTeX Go Rate of Collisional Energy Transfer = Quenching Constant*Quencher Concentration given Degree of Exciplex*Singlet State Concentration

Difference in Acidity between Ground and Excited State

LaTeX Go Difference in pka = pKa of Excited State-pKa of Ground State

Equilibrium Constant for Exciplex Formation

LaTeX Go Equilibrium Constant for Coordinate Complexes = 1/(1-Degree of Exciplex Formation)-1

< Fluoroscence and Phosphorescence Calculators

Initial Intensity given Degree of Exciplex Formation

LaTeX Go Initial Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Fluoroscence Intensity without Quenching

LaTeX Go Intensity Without Quenching = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)

Fluoroscence Intensity

LaTeX Go Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Fluorescence Rate Constant

LaTeX Go Rate Constant of Fluoroscence = Rate of Fluoroscence/Singlet State Concentration

Quenching Concentration Formula

LaTeX Go

Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant
[Q1] = ((Io/I_F)-1)/K_SV

What is concentration fluorescence quenching?

Quenching refers to any process which decreases the fluorescence intensity of a given substance. A variety of processes can result in quenching, such as excited state reactions, energy transfer, complex-formation and collisional quenching. As a consequence, quenching is often heavily dependent on pressure and temperature. Molecular oxygen, iodide ions and acrylamide[1] are common chemical quenchers. The chloride ion is a well known quencher for quinine fluorescence.[2][3][4] Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence.

Quenching is made use of in optode sensors; for instance the quenching effect of oxygen on certain ruthenium complexes allows the measurement of oxygen saturation in solution.

How to Calculate Quenching Concentration?

Quenching Concentration calculator uses Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant to calculate the Quencher Concentration, The Quenching Concentration formula is defined as the concentration of an activator which is higher than an appropriate value (typically several mol%) for which the emission intensity of the phosphor mostly will be decreased. Quencher Concentration is denoted by [Q1] symbol.

How to calculate Quenching Concentration using this online calculator? To use this online calculator for Quenching Concentration, enter Initial Intensity (Io), Fluorosence Intensity (I_F) & Stern Volmner Constant (K_SV) and hit the calculate button. Here is how the Quenching Concentration calculation can be explained with given input values -> -216.666667 = ((500/240)-1)/0.005.

FAQ

What is Quenching Concentration?

The Quenching Concentration formula is defined as the concentration of an activator which is higher than an appropriate value (typically several mol%) for which the emission intensity of the phosphor mostly will be decreased and is represented as [Q1] = ((Io/I_F)-1)/K_SV or Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant. Initial Intensity flux of radiant energy is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy, Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy & Stern Volmner Constant is the bimolecular rate constant of the fluorescence quenching process due to a short-range interaction of species.

How to calculate Quenching Concentration?

The Quenching Concentration formula is defined as the concentration of an activator which is higher than an appropriate value (typically several mol%) for which the emission intensity of the phosphor mostly will be decreased is calculated using Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant. To calculate Quenching Concentration, you need Initial Intensity (Io), Fluorosence Intensity (I_F) & Stern Volmner Constant (K_SV). With our tool, you need to enter the respective value for Initial Intensity, Fluorosence Intensity & Stern Volmner Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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