Modifying Factor of Enzyme Substrate Complex Calculator

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.ⓘ Enzyme Substrate Dissociation Constant [K_i']			+10% -10%

✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Modifying Factor of Enzyme Substrate Complex [α^']

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Modifying Factor of Enzyme Substrate Complex Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)
α^' = 1+(I/K_i')
This formula uses 3 Variables

Variables Used

Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.

STEP 1: Convert Input(s) to Base Unit

Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Dissociation Constant: 15 Mole per Liter --> 15000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

α^' = 1+(I/K_i') --> 1+(9000/15000)

Evaluating ... ...

α^' = 1.6

STEP 3: Convert Result to Output's Unit

1.6 --> No Conversion Required

FINAL ANSWER

1.6 <-- Enzyme Substrate Modifying Factor

(Calculation completed in 00.004 seconds)

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< Enzyme Kinetics Calculators

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Initial Reaction Rate at Low Substrate Concentration terms of Maximum Rate

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< Important Formulas on Enzyme Kinetics Calculators

Initial Reaction Rate given Dissociation Rate Constant

LaTeX Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

LaTeX Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Modifying Factor of Enzyme Substrate Complex

LaTeX Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

LaTeX Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Modifying Factor of Enzyme Substrate Complex Formula

LaTeX Go

Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)
α^' = 1+(I/K_i')

What are the modifying factor of enzyme?

Factors such as pH, temperature, effectors, and inhibitors modify the enzyme conformation, altering its catalytic activity.

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Modifying Factor of Enzyme Substrate Complex?

Modifying Factor of Enzyme Substrate Complex calculator uses Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant) to calculate the Enzyme Substrate Modifying Factor, The Modifying factor of enzyme substrate complex formula is defined as the relation with inhibitor concentration and with the enzyme-substrate dissociation constant. Enzyme Substrate Modifying Factor is denoted by α^' symbol.

How to calculate Modifying Factor of Enzyme Substrate Complex using this online calculator? To use this online calculator for Modifying Factor of Enzyme Substrate Complex, enter Inhibitor Concentration (I) & Enzyme Substrate Dissociation Constant (K_i') and hit the calculate button. Here is how the Modifying Factor of Enzyme Substrate Complex calculation can be explained with given input values -> 1.6 = 1+(9000/15000).

FAQ

What is Modifying Factor of Enzyme Substrate Complex?

The Modifying factor of enzyme substrate complex formula is defined as the relation with inhibitor concentration and with the enzyme-substrate dissociation constant and is represented as α^' = 1+(I/K_i') or Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.

How to calculate Modifying Factor of Enzyme Substrate Complex?

The Modifying factor of enzyme substrate complex formula is defined as the relation with inhibitor concentration and with the enzyme-substrate dissociation constant is calculated using Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant). To calculate Modifying Factor of Enzyme Substrate Complex, you need Inhibitor Concentration (I) & Enzyme Substrate Dissociation Constant (K_i'). With our tool, you need to enter the respective value for Inhibitor Concentration & Enzyme Substrate Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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