Modifying Factor of Enzyme in Michaelis Menten Equation Calculator

✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Enzyme Substrate Modifying Factor [α^']			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%

✖The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.ⓘ Modifying Factor of Enzyme in Michaelis Menten Equation [α]

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Modifying Factor of Enzyme in Michaelis Menten Equation Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant
α = (((V_max*S)/V₀)-(α^'*S))/K_M
This formula uses 6 Variables

Variables Used

Enzyme Modifying Factor - The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

STEP 1: Convert Input(s) to Base Unit

Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

α = (((V_max*S)/V₀)-(α^'*S))/K_M --> (((40000*1500)/450)-(2*1500))/3000

Evaluating ... ...

α = 43.4444444444444

STEP 3: Convert Result to Output's Unit

43.4444444444444 --> No Conversion Required

FINAL ANSWER

43.4444444444444 ≈ 43.44444 <-- Enzyme Modifying Factor

(Calculation completed in 00.004 seconds)

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< Michaelis Menten Kinetics Equation Calculators

Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate

Maximum Rate of System from Michaelis Menten Kinetics equation

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/Substrate Concentration

Substrate Concentration from Michaelis Menten Kinetics Equation

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Michaelis Constant from Michaelis Menten kinetics equation

LaTeX Go Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)

Modifying Factor of Enzyme in Michaelis Menten Equation Formula

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What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Modifying Factor of Enzyme in Michaelis Menten Equation?

Modifying Factor of Enzyme in Michaelis Menten Equation calculator uses Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant to calculate the Enzyme Modifying Factor, The Modifying factor of enzyme in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation. Enzyme Modifying Factor is denoted by α symbol.

How to calculate Modifying Factor of Enzyme in Michaelis Menten Equation using this online calculator? To use this online calculator for Modifying Factor of Enzyme in Michaelis Menten Equation, enter Maximum Rate (V_max), Substrate Concentration (S), Initial Reaction Rate (V₀), Enzyme Substrate Modifying Factor (α^') & Michaelis Constant (K_M) and hit the calculate button. Here is how the Modifying Factor of Enzyme in Michaelis Menten Equation calculation can be explained with given input values -> 43.44444 = (((40000*1500)/450)-(2*1500))/3000.

FAQ

What is Modifying Factor of Enzyme in Michaelis Menten Equation?

The Modifying factor of enzyme in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation and is represented as α = (((V_max*S)/V₀)-(α^'*S))/K_M or Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant. The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Substrate Concentration is the number of moles of substrate per liter solution, The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place, The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex & The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

How to calculate Modifying Factor of Enzyme in Michaelis Menten Equation?

The Modifying factor of enzyme in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation is calculated using Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant. To calculate Modifying Factor of Enzyme in Michaelis Menten Equation, you need Maximum Rate (V_max), Substrate Concentration (S), Initial Reaction Rate (V₀), Enzyme Substrate Modifying Factor (α^') & Michaelis Constant (K_M). With our tool, you need to enter the respective value for Maximum Rate, Substrate Concentration, Initial Reaction Rate, Enzyme Substrate Modifying Factor & Michaelis Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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