Michaelis Constant in Presence of Uncompetitive Inhibitor Calculator

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Enzyme Substrate Modifying Factor [α^']			+10% -10%

✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant in Presence of Uncompetitive Inhibitor [K_M]

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Michaelis Constant in Presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate
K_M = (S*(V_max-(V₀*α^')))/V₀
This formula uses 5 Variables

Variables Used

Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

STEP 1: Convert Input(s) to Base Unit

Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_M = (S*(V_max-(V₀*α^')))/V₀ --> (1500*(40000-(450*2)))/450

Evaluating ... ...

K_M = 130333.333333333

STEP 3: Convert Result to Output's Unit

130333.333333333 Mole per Cubic Meter -->130.333333333333 Mole per Liter (Check conversion here)

FINAL ANSWER

130.333333333333 ≈ 130.3333 Mole per Liter <-- Michaelis Constant

(Calculation completed in 00.020 seconds)

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< Uncompetitive Inhibitor Calculators

Initial Reaction Rate in presence of Uncompetitive Inhibitor

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

LaTeX Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Michaelis Constant in Presence of Uncompetitive Inhibitor Formula

LaTeX Go

Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate
K_M = (S*(V_max-(V₀*α^')))/V₀

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Michaelis Constant in Presence of Uncompetitive Inhibitor?

Michaelis Constant in Presence of Uncompetitive Inhibitor calculator uses Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate to calculate the Michaelis Constant, The Michaelis constant in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation. Michaelis Constant is denoted by K_M symbol.

How to calculate Michaelis Constant in Presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Michaelis Constant in Presence of Uncompetitive Inhibitor, enter Substrate Concentration (S), Maximum Rate (V_max), Initial Reaction Rate (V₀) & Enzyme Substrate Modifying Factor (α^') and hit the calculate button. Here is how the Michaelis Constant in Presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 0.130333 = (1500*(40000-(450*2)))/450.

FAQ

What is Michaelis Constant in Presence of Uncompetitive Inhibitor?

The Michaelis constant in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation and is represented as K_M = (S*(V_max-(V₀*α^')))/V₀ or Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate. The Substrate Concentration is the number of moles of substrate per liter solution, The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place & The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

How to calculate Michaelis Constant in Presence of Uncompetitive Inhibitor?

The Michaelis constant in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation is calculated using Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate. To calculate Michaelis Constant in Presence of Uncompetitive Inhibitor, you need Substrate Concentration (S), Maximum Rate (V_max), Initial Reaction Rate (V₀) & Enzyme Substrate Modifying Factor (α^'). With our tool, you need to enter the respective value for Substrate Concentration, Maximum Rate, Initial Reaction Rate & Enzyme Substrate Modifying Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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