Michaelis Constant given Maximum Rate at Low Substrate Concentration Calculator

✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%

✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant given Maximum Rate at Low Substrate Concentration [K_M]

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Michaelis Constant given Maximum Rate at Low Substrate Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate
K_M = (V_max*S)/V₀
This formula uses 4 Variables

Variables Used

Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

STEP 1: Convert Input(s) to Base Unit

Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_M = (V_max*S)/V₀ --> (40000*1500)/450

Evaluating ... ...

K_M = 133333.333333333

STEP 3: Convert Result to Output's Unit

133333.333333333 Mole per Cubic Meter -->133.333333333333 Mole per Liter (Check conversion here)

FINAL ANSWER

133.333333333333 ≈ 133.3333 Mole per Liter <-- Michaelis Constant

(Calculation completed in 00.004 seconds)

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LaTeX Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate

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Michaelis Constant from Michaelis Menten kinetics equation

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Michaelis Constant given Maximum Rate at Low Substrate Concentration Formula

LaTeX Go

Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate
K_M = (V_max*S)/V₀

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Michaelis Constant given Maximum Rate at Low Substrate Concentration?

Michaelis Constant given Maximum Rate at Low Substrate Concentration calculator uses Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate to calculate the Michaelis Constant, The Michaelis constant given maximum rate at low substrate concentration formula is defined as the relation with the initial and maximum rate of the system and with the substrate concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Michaelis Constant is denoted by K_M symbol.

How to calculate Michaelis Constant given Maximum Rate at Low Substrate Concentration using this online calculator? To use this online calculator for Michaelis Constant given Maximum Rate at Low Substrate Concentration, enter Maximum Rate (V_max), Substrate Concentration (S) & Initial Reaction Rate (V₀) and hit the calculate button. Here is how the Michaelis Constant given Maximum Rate at Low Substrate Concentration calculation can be explained with given input values -> 0.133333 = (40000*1500)/450.

FAQ

What is Michaelis Constant given Maximum Rate at Low Substrate Concentration?

The Michaelis constant given maximum rate at low substrate concentration formula is defined as the relation with the initial and maximum rate of the system and with the substrate concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant and is represented as K_M = (V_max*S)/V₀ or Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate. The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Substrate Concentration is the number of moles of substrate per liter solution & The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

How to calculate Michaelis Constant given Maximum Rate at Low Substrate Concentration?

The Michaelis constant given maximum rate at low substrate concentration formula is defined as the relation with the initial and maximum rate of the system and with the substrate concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant is calculated using Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate. To calculate Michaelis Constant given Maximum Rate at Low Substrate Concentration, you need Maximum Rate (V_max), Substrate Concentration (S) & Initial Reaction Rate (V₀). With our tool, you need to enter the respective value for Maximum Rate, Substrate Concentration & Initial Reaction Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Michaelis Constant?

In this formula, Michaelis Constant uses Maximum Rate, Substrate Concentration & Initial Reaction Rate. We can use 3 other way(s) to calculate the same, which is/are as follows -

Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)
Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate
Michaelis Constant = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate

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