LCM of two numbers

Lineweaver Burk plot Calculator

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✖The maximum reaction rate is the maximum rate occurs in a chemical reaction.ⓘ The maximum reaction rate [V_max]			+10% -10%
✖The substrare concentration is the concentration of the substrate which undergoes enzyme treatment.ⓘ The substrare concentration [S_conc.]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%

✖The initial reaction rate is the rate at which a chemical reaction is procceding at the very beggining, at the moment when the reactants are mixed.ⓘ Lineweaver Burk plot [V_O]

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Formula

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Lineweaver Burk plot

Formula

V O = \frac{V max \cdot S conc.}{K M + S conc.}

Example

0.198675 mol/L*s = \frac{30 mol/L*s \cdot 20 g/L}{3 mol/L + 20 g/L}

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Lineweaver Burk plot Solution

STEP 0: Pre-Calculation Summary

Formula Used

The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration)
V_O = (V_max*S_conc.)/(K_M+S_conc.)
This formula uses 4 Variables

Variables Used

The initial reaction rate - (Measured in Mole per Cubic Meter Second) - The initial reaction rate is the rate at which a chemical reaction is procceding at the very beggining, at the moment when the reactants are mixed.
The maximum reaction rate - (Measured in Mole per Cubic Meter Second) - The maximum reaction rate is the maximum rate occurs in a chemical reaction.
The substrare concentration - (Measured in Kilogram per Cubic Meter) - The substrare concentration is the concentration of the substrate which undergoes enzyme treatment.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

STEP 1: Convert Input(s) to Base Unit

The maximum reaction rate: 30 Mole per Liter Second --> 30000 Mole per Cubic Meter Second (Check conversion here)
The substrare concentration: 20 Gram per Liter --> 20 Kilogram per Cubic Meter (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V_O = (V_max*S_conc.)/(K_M+S_conc.) --> (30000*20)/(3000+20)

Evaluating ... ...

V_O = 198.675496688742

STEP 3: Convert Result to Output's Unit

198.675496688742 Mole per Cubic Meter Second -->0.198675496688742 Mole per Liter Second (Check conversion here)

FINAL ANSWER

0.198675496688742 ≈ 0.198675 Mole per Liter Second <-- The initial reaction rate

(Calculation completed in 00.004 seconds)

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Created by harykrishnan

SRM Institute of Science and Technology (SRMIST), Chennai

harykrishnan has created this Calculator and 10+ more calculators!

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National University of Judicial Science (NUJS), Kolkata

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Lineweaver Burk plot Formula

The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration)
V_O = (V_max*S_conc.)/(K_M+S_conc.)

What is Lineweaver Burk plot?

Lineweaver Burk plot is applied to determine the kinetic parameters due to its simplicity, it is derived from Michaelis-Menten equation which is been used to determine these kinetic parameters with high precision.

How to Calculate Lineweaver Burk plot?

Lineweaver Burk plot calculator uses The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration) to calculate the The initial reaction rate, Lineweaver Burk plot also known as the double reciprocal plot, is a graphical method used to analyze enzyme kinetics data. It provides a linear representation of the Michaelis-Menten equation, making it easier to determine important kinetic parameters of enzyme-catalyzed reactions. The initial reaction rate is denoted by V_O symbol.

How to calculate Lineweaver Burk plot using this online calculator? To use this online calculator for Lineweaver Burk plot, enter The maximum reaction rate (V_max), The substrare concentration (S_conc.) & Michaelis Constant (K_M) and hit the calculate button. Here is how the Lineweaver Burk plot calculation can be explained with given input values -> 0.000199 = (30000*20)/(3000+20).

FAQ

What is Lineweaver Burk plot?

Lineweaver Burk plot also known as the double reciprocal plot, is a graphical method used to analyze enzyme kinetics data. It provides a linear representation of the Michaelis-Menten equation, making it easier to determine important kinetic parameters of enzyme-catalyzed reactions and is represented as V_O = (V_max*S_conc.)/(K_M+S_conc.) or The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration). The maximum reaction rate is the maximum rate occurs in a chemical reaction, The substrare concentration is the concentration of the substrate which undergoes enzyme treatment & The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

How to calculate Lineweaver Burk plot?

Lineweaver Burk plot also known as the double reciprocal plot, is a graphical method used to analyze enzyme kinetics data. It provides a linear representation of the Michaelis-Menten equation, making it easier to determine important kinetic parameters of enzyme-catalyzed reactions is calculated using The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration). To calculate Lineweaver Burk plot, you need The maximum reaction rate (V_max), The substrare concentration (S_conc.) & Michaelis Constant (K_M). With our tool, you need to enter the respective value for The maximum reaction rate, The substrare concentration & Michaelis Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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