What is concentration fluorescence quenching?
Quenching refers to any process which decreases the fluorescence intensity of a given substance. A variety of processes can result in quenching, such as excited state reactions, energy transfer, complex-formation and collisional quenching. As a consequence, quenching is often heavily dependent on pressure and temperature. Molecular oxygen, iodide ions and acrylamide are common chemical quenchers. The chloride ion is a well known quencher for quinine fluorescence. Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence.
Quenching is made use of in optode sensors; for instance the quenching effect of oxygen on certain ruthenium complexes allows the measurement of oxygen saturation in solution.
How to Calculate Lifetime Ratio?
Lifetime Ratio calculator uses Lifetime Ratio = 1+(Quenching Constant*Singlet Life time given Degree of Exciplex*Quencher Concentration given Degree of Exciplex) to calculate the Lifetime Ratio, Lifetime Ratio is the ratio of singlet state lifetime without quencher to singlet state lifetime with quencher of a given system. Lifetime Ratio is denoted by Ratioζ symbol.
How to calculate Lifetime Ratio using this online calculator? To use this online calculator for Lifetime Ratio, enter Quenching Constant (Kq), Singlet Life time given Degree of Exciplex (ζo) & Quencher Concentration given Degree of Exciplex ([Q]) and hit the calculate button. Here is how the Lifetime Ratio calculation can be explained with given input values -> 1.0108 = 1+(6*0.8*1.5).