Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation Calculator

✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.ⓘ Enzyme Modifying Factor [α]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Enzyme Substrate Modifying Factor [α^']			+10% -10%

✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation [V₀]

⎘ Copy

👎

Formula

✖

Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation

Formula

V 0 = \frac{V max \cdot S}{(α \cdot K M) + (α' \cdot S)}

Example

3.333333 mol/L*s = \frac{40 mol/L*s \cdot 1.5 mol/L}{(5 \cdot 3 mol/L) + (2 \cdot 1.5 mol/L)}

Calculator

LaTeX

Reset

👍

Download Chemical Kinetics Formula PDF PDF Image

Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation Solution

STEP 0: Pre-Calculation Summary

Formula Used

Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration))
V₀ = (V_max*S)/((α*K_M)+(α^'*S))
This formula uses 6 Variables

Variables Used

Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Enzyme Modifying Factor - The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

STEP 1: Convert Input(s) to Base Unit

Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Enzyme Modifying Factor: 5 --> No Conversion Required
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V₀ = (V_max*S)/((α*K_M)+(α^'*S)) --> (40000*1500)/((5*3000)+(2*1500))

Evaluating ... ...

V₀ = 3333.33333333333

STEP 3: Convert Result to Output's Unit

3333.33333333333 Mole per Cubic Meter Second -->3.33333333333333 Mole per Liter Second (Check conversion here)

FINAL ANSWER

3.33333333333333 ≈ 3.333333 Mole per Liter Second <-- Initial Reaction Rate

(Calculation completed in 00.004 seconds)

You are here -

Home » Chemistry » Chemical Kinetics » Enzyme Kinetics » Michaelis Menten Kinetics Equation » Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation

Credits

Created by Prashant Singh

K J Somaiya College of science (K J Somaiya), Mumbai

Prashant Singh has created this Calculator and 700+ more calculators!

Verified by Prerana Bakli

University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA

Prerana Bakli has verified this Calculator and 1600+ more calculators!

< Michaelis Menten Kinetics Equation Calculators

Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate

Maximum Rate of System from Michaelis Menten Kinetics equation

Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/Substrate Concentration

Substrate Concentration from Michaelis Menten Kinetics Equation

Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Michaelis Constant from Michaelis Menten kinetics equation

Go Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)

Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation Formula

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation?

Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation calculator uses Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration)) to calculate the Initial Reaction Rate, The Initial reaction rate of enzyme given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation. Initial Reaction Rate is denoted by V₀ symbol.

How to calculate Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation using this online calculator? To use this online calculator for Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation, enter Maximum Rate (V_max), Substrate Concentration (S), Enzyme Modifying Factor (α), Michaelis Constant (K_M) & Enzyme Substrate Modifying Factor (α^') and hit the calculate button. Here is how the Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation calculation can be explained with given input values -> 0.003333 = (40000*1500)/((5*3000)+(2*1500)).

FAQ

What is Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation?

The Initial reaction rate of enzyme given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation and is represented as V₀ = (V_max*S)/((α*K_M)+(α^'*S)) or Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration)). The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Substrate Concentration is the number of moles of substrate per liter solution, The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system & The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

How to calculate Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation?

The Initial reaction rate of enzyme given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation is calculated using Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration)). To calculate Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation, you need Maximum Rate (V_max), Substrate Concentration (S), Enzyme Modifying Factor (α), Michaelis Constant (K_M) & Enzyme Substrate Modifying Factor (α^'). With our tool, you need to enter the respective value for Maximum Rate, Substrate Concentration, Enzyme Modifying Factor, Michaelis Constant & Enzyme Substrate Modifying Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Initial Reaction Rate?

In this formula, Initial Reaction Rate uses Maximum Rate, Substrate Concentration, Enzyme Modifying Factor, Michaelis Constant & Enzyme Substrate Modifying Factor. We can use 1 other way(s) to calculate the same, which is/are as follows -

Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Apparent Michaelis Constant+Substrate Concentration)

Home

FREE PDFs

🔍 Search