Initial Reaction Rate at Low Substrate Concentration Calculator

✖The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.ⓘ Catalytic Rate Constant [k_cat]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%

✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate at Low Substrate Concentration [V₀]

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Initial Reaction Rate at Low Substrate Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant
V₀ = (k_cat*[E₀]*S)/K_M
This formula uses 5 Variables

Variables Used

Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

STEP 1: Convert Input(s) to Base Unit

Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V₀ = (k_cat*[E₀]*S)/K_M --> (0.65*100000*1500)/3000

Evaluating ... ...

V₀ = 32500

STEP 3: Convert Result to Output's Unit

32500 Mole per Cubic Meter Second -->32.5 Mole per Liter Second (Check conversion here)

FINAL ANSWER

32.5 Mole per Liter Second <-- Initial Reaction Rate

(Calculation completed in 00.020 seconds)

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< Enzyme Kinetics Calculators

Initial Reaction Rate given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)

Initial Reaction Rate at Low Substrate Concentration

LaTeX Go Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant

Initial Reaction Rate in Michaelis Menten kinetics Equation

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)

Initial Reaction Rate at Low Substrate Concentration terms of Maximum Rate

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/Michaelis Constant

Initial Reaction Rate at Low Substrate Concentration Formula

LaTeX Go

Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant
V₀ = (k_cat*[E₀]*S)/K_M

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Initial Reaction Rate at Low Substrate Concentration?

Initial Reaction Rate at Low Substrate Concentration calculator uses Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant to calculate the Initial Reaction Rate, The Initial reaction rate at low substrate concentration formula is defined as the relation with initial enzyme concentration and the concentration of a substrate. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Initial Reaction Rate is denoted by V₀ symbol.

How to calculate Initial Reaction Rate at Low Substrate Concentration using this online calculator? To use this online calculator for Initial Reaction Rate at Low Substrate Concentration, enter Catalytic Rate Constant (k_cat), Initial Enzyme Concentration ([E₀]), Substrate Concentration (S) & Michaelis Constant (K_M) and hit the calculate button. Here is how the Initial Reaction Rate at Low Substrate Concentration calculation can be explained with given input values -> 0.0325 = (0.65*100000*1500)/3000.

FAQ

What is Initial Reaction Rate at Low Substrate Concentration?

The Initial reaction rate at low substrate concentration formula is defined as the relation with initial enzyme concentration and the concentration of a substrate. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant and is represented as V₀ = (k_cat*[E₀]*S)/K_M or Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant. The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product, The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Substrate Concentration is the number of moles of substrate per liter solution & The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

How to calculate Initial Reaction Rate at Low Substrate Concentration?

The Initial reaction rate at low substrate concentration formula is defined as the relation with initial enzyme concentration and the concentration of a substrate. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant is calculated using Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant. To calculate Initial Reaction Rate at Low Substrate Concentration, you need Catalytic Rate Constant (k_cat), Initial Enzyme Concentration ([E₀]), Substrate Concentration (S) & Michaelis Constant (K_M). With our tool, you need to enter the respective value for Catalytic Rate Constant, Initial Enzyme Concentration, Substrate Concentration & Michaelis Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Initial Reaction Rate?

In this formula, Initial Reaction Rate uses Catalytic Rate Constant, Initial Enzyme Concentration, Substrate Concentration & Michaelis Constant. We can use 3 other way(s) to calculate the same, which is/are as follows -

Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)
Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)
Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/Michaelis Constant

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