Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Calculator

✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.ⓘ Catalytic Rate Constant [k_cat]			+10% -10%

✖Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant [[E_initial]]

⎘ Copy

👎

Formula

LaTeX

Reset

👍

Download Chemical Kinetics Formula PDF PDF Image

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant
[E_initial] = V_max/k_cat
This formula uses 3 Variables

Variables Used

Enzyme Concentration Initially - (Measured in Mole per Cubic Meter) - Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

STEP 1: Convert Input(s) to Base Unit

Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

[E_initial] = V_max/k_cat --> 40000/0.65

Evaluating ... ...

[E_initial] = 61538.4615384615

STEP 3: Convert Result to Output's Unit

61538.4615384615 Mole per Cubic Meter -->61.5384615384615 Mole per Liter (Check conversion here)

FINAL ANSWER

61.5384615384615 ≈ 61.53846 Mole per Liter <-- Enzyme Concentration Initially

(Calculation completed in 00.004 seconds)

You are here -

Home » Chemistry » Chemical Kinetics » Enzyme Kinetics » Michaelis Menten Kinetics Equation » Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

Credits

Created by Prashant Singh

K J Somaiya College of science (K J Somaiya), Mumbai

Prashant Singh has created this Calculator and 700+ more calculators!

Verified by Prerana Bakli

University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA

Prerana Bakli has verified this Calculator and 1600+ more calculators!

< Michaelis Menten Kinetics Equation Calculators

Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate

Maximum Rate of System from Michaelis Menten Kinetics equation

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/Substrate Concentration

Substrate Concentration from Michaelis Menten Kinetics Equation

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Michaelis Constant from Michaelis Menten kinetics equation

LaTeX Go Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)

< Important Formulas on Enzyme Kinetics Calculators

Initial Reaction Rate given Dissociation Rate Constant

LaTeX Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

LaTeX Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Modifying Factor of Enzyme Substrate Complex

LaTeX Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

LaTeX Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Formula

LaTeX Go

Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant
[E_initial] = V_max/k_cat

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant calculator uses Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant to calculate the Enzyme Concentration Initially, The Initial enzyme concentration if substrate concentration is higher than Michaelis constant formula is defined as the ratio of the maximum rate to the catalytic rate constant. Here substrate concentration is very large in comparison to Michaelis constant. Enzyme Concentration Initially is denoted by [E_initial] symbol.

How to calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant using this online calculator? To use this online calculator for Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant, enter Maximum Rate (V_max) & Catalytic Rate Constant (k_cat) and hit the calculate button. Here is how the Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant calculation can be explained with given input values -> 0.061538 = 40000/0.65.

FAQ

What is Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

The Initial enzyme concentration if substrate concentration is higher than Michaelis constant formula is defined as the ratio of the maximum rate to the catalytic rate constant. Here substrate concentration is very large in comparison to Michaelis constant and is represented as [E_initial] = V_max/k_cat or Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant. The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration & The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

How to calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

The Initial enzyme concentration if substrate concentration is higher than Michaelis constant formula is defined as the ratio of the maximum rate to the catalytic rate constant. Here substrate concentration is very large in comparison to Michaelis constant is calculated using Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant. To calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant, you need Maximum Rate (V_max) & Catalytic Rate Constant (k_cat). With our tool, you need to enter the respective value for Maximum Rate & Catalytic Rate Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Concentration Initially?

In this formula, Enzyme Concentration Initially uses Maximum Rate & Catalytic Rate Constant. We can use 2 other way(s) to calculate the same, which is/are as follows -

Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)
Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)

Home

FREE PDFs

🔍 Search