Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law Calculator

✖The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.ⓘ Catalyst Concentration [E]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Enzyme Inhibitor Complex Concentration is the number of moles of enzyme inhibitor substrate complex per liter of the enzymatic solution.ⓘ Enzyme Inhibitor Complex Concentration [EI]			+10% -10%

✖Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law [[E_initial]]

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Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)
[E_initial] = (E+ES+EI)
This formula uses 4 Variables

Variables Used

Enzyme Concentration Initially - (Measured in Mole per Cubic Meter) - Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction.
Catalyst Concentration - (Measured in Mole per Cubic Meter) - The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Enzyme Inhibitor Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Complex Concentration is the number of moles of enzyme inhibitor substrate complex per liter of the enzymatic solution.

STEP 1: Convert Input(s) to Base Unit

Catalyst Concentration: 25 Mole per Liter --> 25000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Complex Concentration: 29 Mole per Liter --> 29000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

[E_initial] = (E+ES+EI) --> (25000+10000+29000)

Evaluating ... ...

[E_initial] = 64000

STEP 3: Convert Result to Output's Unit

64000 Mole per Cubic Meter -->64 Mole per Liter (Check conversion here)

FINAL ANSWER

64 Mole per Liter <-- Enzyme Concentration Initially

(Calculation completed in 00.020 seconds)

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< Enzyme Conservation Law Calculators

Concentration of Enzyme Catalyst in Presence of Inhibitor by Enzyme Conservation Law

LaTeX Go Catalyst Concentration = (Initial Enzyme Concentration-Enzyme Substrate Complex Concentration-Enzyme Inhibitor Complex Concentration)

Concentration of Enzyme Substrate Complex from Enzyme Conservation Law

LaTeX Go Enzyme Substrate Complex Concentration = Initial Enzyme Concentration-Catalyst Concentration

Initial Concentration of Enzyme from Enzyme Conservation Law

LaTeX Go Initial Enzyme Concentration = Catalyst Concentration+Enzyme Substrate Complex Concentration

Concentration of Enzyme Catalyst by Enzyme Conservation Law

LaTeX Go Catalyst Concentration = Initial Enzyme Concentration-Enzyme Substrate Complex Concentration

< Important Formulas on Enzyme Kinetics Calculators

Initial Reaction Rate given Dissociation Rate Constant

LaTeX Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

LaTeX Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Modifying Factor of Enzyme Substrate Complex

LaTeX Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

LaTeX Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law Formula

LaTeX Go

Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)
[E_initial] = (E+ES+EI)

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law?

Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law calculator uses Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration) to calculate the Enzyme Concentration Initially, The Initial concentration of enzyme in presence of inhibitor by enzyme conservation law formula is defined as the submission of the concentration of each of the enzyme species. Enzyme Concentration Initially is denoted by [E_initial] symbol.

How to calculate Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law using this online calculator? To use this online calculator for Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law, enter Catalyst Concentration (E), Enzyme Substrate Complex Concentration (ES) & Enzyme Inhibitor Complex Concentration (EI) and hit the calculate button. Here is how the Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law calculation can be explained with given input values -> 0.064 = (25000+10000+29000).

FAQ

What is Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law?

The Initial concentration of enzyme in presence of inhibitor by enzyme conservation law formula is defined as the submission of the concentration of each of the enzyme species and is represented as [E_initial] = (E+ES+EI) or Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration). The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate & The Enzyme Inhibitor Complex Concentration is the number of moles of enzyme inhibitor substrate complex per liter of the enzymatic solution.

How to calculate Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law?

The Initial concentration of enzyme in presence of inhibitor by enzyme conservation law formula is defined as the submission of the concentration of each of the enzyme species is calculated using Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration). To calculate Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law, you need Catalyst Concentration (E), Enzyme Substrate Complex Concentration (ES) & Enzyme Inhibitor Complex Concentration (EI). With our tool, you need to enter the respective value for Catalyst Concentration, Enzyme Substrate Complex Concentration & Enzyme Inhibitor Complex Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Concentration Initially?

In this formula, Enzyme Concentration Initially uses Catalyst Concentration, Enzyme Substrate Complex Concentration & Enzyme Inhibitor Complex Concentration. We can use 2 other way(s) to calculate the same, which is/are as follows -

Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)
Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant

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