Inhibitor's Dissociation Constant given Michaelis Menten Constant Calculator

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.ⓘ Apparent Michaelis Constant [Km^app]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%

✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Inhibitor's Dissociation Constant given Michaelis Menten Constant [K_i]

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Inhibitor's Dissociation Constant given Michaelis Menten Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1))
K_i = (I/((Km^app/K_M)-1))
This formula uses 4 Variables

Variables Used

Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Apparent Michaelis Constant - (Measured in Mole per Cubic Meter) - The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

STEP 1: Convert Input(s) to Base Unit

Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Apparent Michaelis Constant: 12 Mole per Liter --> 12000 Mole per Cubic Meter (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_i = (I/((Km^app/K_M)-1)) --> (9000/((12000/3000)-1))

Evaluating ... ...

K_i = 3000

STEP 3: Convert Result to Output's Unit

3000 Mole per Cubic Meter -->3 Mole per Liter (Check conversion here)

FINAL ANSWER

3 Mole per Liter <-- Enzyme Inhibitor Dissociation Constant

(Calculation completed in 00.004 seconds)

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< Michaelis Menten Kinetics Equation Calculators

Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

LaTeX Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate

Maximum Rate of System from Michaelis Menten Kinetics equation

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/Substrate Concentration

Substrate Concentration from Michaelis Menten Kinetics Equation

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Michaelis Constant from Michaelis Menten kinetics equation

LaTeX Go Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)

Inhibitor's Dissociation Constant given Michaelis Menten Constant Formula

LaTeX Go

Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1))
K_i = (I/((Km^app/K_M)-1))

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Inhibitor's Dissociation Constant given Michaelis Menten Constant?

Inhibitor's Dissociation Constant given Michaelis Menten Constant calculator uses Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1)) to calculate the Enzyme Inhibitor Dissociation Constant, The Inhibitor's dissociation constant given Michaelis Menten constant formula is defined as the relation with Michaelis constant and apparent Michaelis constant and with the inhibitor concentration. Enzyme Inhibitor Dissociation Constant is denoted by K_i symbol.

How to calculate Inhibitor's Dissociation Constant given Michaelis Menten Constant using this online calculator? To use this online calculator for Inhibitor's Dissociation Constant given Michaelis Menten Constant, enter Inhibitor Concentration (I), Apparent Michaelis Constant (Km^app) & Michaelis Constant (K_M) and hit the calculate button. Here is how the Inhibitor's Dissociation Constant given Michaelis Menten Constant calculation can be explained with given input values -> 0.003 = (9000/((12000/3000)-1)).

FAQ

What is Inhibitor's Dissociation Constant given Michaelis Menten Constant?

The Inhibitor's dissociation constant given Michaelis Menten constant formula is defined as the relation with Michaelis constant and apparent Michaelis constant and with the inhibitor concentration and is represented as K_i = (I/((Km^app/K_M)-1)) or Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1)). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system, The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor & The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

How to calculate Inhibitor's Dissociation Constant given Michaelis Menten Constant?

The Inhibitor's dissociation constant given Michaelis Menten constant formula is defined as the relation with Michaelis constant and apparent Michaelis constant and with the inhibitor concentration is calculated using Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1)). To calculate Inhibitor's Dissociation Constant given Michaelis Menten Constant, you need Inhibitor Concentration (I), Apparent Michaelis Constant (Km^app) & Michaelis Constant (K_M). With our tool, you need to enter the respective value for Inhibitor Concentration, Apparent Michaelis Constant & Michaelis Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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