Inhibitor Concentration in Presence of Uncompetitive Inhibitor Calculator

✖The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.ⓘ Enzyme Substrate Dissociation Constant [K_i']			+10% -10%
✖The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.ⓘ Enzyme Substrate Inhibitor Complex Concentration [ESI]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration in Presence of Uncompetitive Inhibitor [I]

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Inhibitor Concentration in Presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration
I = (K_i'*ESI)/ES
This formula uses 4 Variables

Variables Used

Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.
Enzyme Substrate Inhibitor Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

STEP 1: Convert Input(s) to Base Unit

Enzyme Substrate Dissociation Constant: 15 Mole per Liter --> 15000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Inhibitor Complex Concentration: 2.5 Mole per Liter --> 2500 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

I = (K_i'*ESI)/ES --> (15000*2500)/10000

Evaluating ... ...

I = 3750

STEP 3: Convert Result to Output's Unit

3750 Mole per Cubic Meter -->3.75 Mole per Liter (Check conversion here)

FINAL ANSWER

3.75 Mole per Liter <-- Inhibitor Concentration

(Calculation completed in 00.020 seconds)

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< Uncompetitive Inhibitor Calculators

Initial Reaction Rate in presence of Uncompetitive Inhibitor

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

LaTeX Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Inhibitor Concentration in Presence of Uncompetitive Inhibitor Formula

LaTeX Go

Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration
I = (K_i'*ESI)/ES

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor?

Inhibitor Concentration in Presence of Uncompetitive Inhibitor calculator uses Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration to calculate the Inhibitor Concentration, The Inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species. Inhibitor Concentration is denoted by I symbol.

How to calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Inhibitor Concentration in Presence of Uncompetitive Inhibitor, enter Enzyme Substrate Dissociation Constant (K_i'), Enzyme Substrate Inhibitor Complex Concentration (ESI) & Enzyme Substrate Complex Concentration (ES) and hit the calculate button. Here is how the Inhibitor Concentration in Presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 0.00375 = (15000*2500)/10000.

FAQ

What is Inhibitor Concentration in Presence of Uncompetitive Inhibitor?

The Inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species and is represented as I = (K_i'*ESI)/ES or Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration. The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product, The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex & The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

How to calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor?

The Inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species is calculated using Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration. To calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor, you need Enzyme Substrate Dissociation Constant (K_i'), Enzyme Substrate Inhibitor Complex Concentration (ESI) & Enzyme Substrate Complex Concentration (ES). With our tool, you need to enter the respective value for Enzyme Substrate Dissociation Constant, Enzyme Substrate Inhibitor Complex Concentration & Enzyme Substrate Complex Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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