Inhibitor Concentration given Apparent Initial Enzyme Concentration Calculator

✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor.ⓘ Apparent Initial Enzyme Concentration [E0^app]			+10% -10%
✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Enzyme Inhibitor Dissociation Constant [K_i]			+10% -10%

✖Inhibitor Concentration for CI is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration given Apparent Initial Enzyme Concentration [I_CI]

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Inhibitor Concentration given Apparent Initial Enzyme Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant
I_CI = (([E₀]/E0^app)-1)*K_i
This formula uses 4 Variables

Variables Used

Inhibitor Concentration for CI - (Measured in Mole per Cubic Meter) - Inhibitor Concentration for CI is defined as the number of moles of inhibitor present per liter of solution of the system.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Apparent Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

STEP 1: Convert Input(s) to Base Unit

Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Apparent Initial Enzyme Concentration: 0.06 Mole per Liter --> 60 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

I_CI = (([E₀]/E0^app)-1)*K_i --> ((100000/60)-1)*19000

Evaluating ... ...

I_CI = 31647666.6666667

STEP 3: Convert Result to Output's Unit

31647666.6666667 Mole per Cubic Meter -->31647.6666666667 Mole per Liter (Check conversion here)

FINAL ANSWER

31647.6666666667 ≈ 31647.67 Mole per Liter <-- Inhibitor Concentration for CI

(Calculation completed in 00.011 seconds)

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Inhibitor Concentration given Apparent Initial Enzyme Concentration Formula

LaTeX Go

Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant
I_CI = (([E₀]/E0^app)-1)*K_i

What is Non-competitive inhibition?

Non-competitive inhibition is a type of enzyme inhibition where the inhibitor reduces the activity of the enzyme and binds equally well to the enzyme whether or not it has already bound the substrate. In the presence of a non-competitive inhibitor, the apparent enzyme affinity is equivalent to the actual affinity because the inhibitor binds to both the enzyme and the enzyme-substrate complex equally so that the equilibrium is maintained. However, since some enzyme is always inhibited from converting the substrate to product, the effective enzyme concentration is lowered.

How to Calculate Inhibitor Concentration given Apparent Initial Enzyme Concentration?

Inhibitor Concentration given Apparent Initial Enzyme Concentration calculator uses Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant to calculate the Inhibitor Concentration for CI, The Inhibitor concentration given apparent initial enzyme concentration formula is defined as the relation with the initial enzyme concentration, apparent initial enzyme concentration, and with a dissociation constant. Inhibitor Concentration for CI is denoted by I_CI symbol.

How to calculate Inhibitor Concentration given Apparent Initial Enzyme Concentration using this online calculator? To use this online calculator for Inhibitor Concentration given Apparent Initial Enzyme Concentration, enter Initial Enzyme Concentration ([E₀]), Apparent Initial Enzyme Concentration (E0^app) & Enzyme Inhibitor Dissociation Constant (K_i) and hit the calculate button. Here is how the Inhibitor Concentration given Apparent Initial Enzyme Concentration calculation can be explained with given input values -> 31.64767 = ((100000/60)-1)*19000.

FAQ

What is Inhibitor Concentration given Apparent Initial Enzyme Concentration?

The Inhibitor concentration given apparent initial enzyme concentration formula is defined as the relation with the initial enzyme concentration, apparent initial enzyme concentration, and with a dissociation constant and is represented as I_CI = (([E₀]/E0^app)-1)*K_i or Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant. The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor & The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

How to calculate Inhibitor Concentration given Apparent Initial Enzyme Concentration?

The Inhibitor concentration given apparent initial enzyme concentration formula is defined as the relation with the initial enzyme concentration, apparent initial enzyme concentration, and with a dissociation constant is calculated using Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant. To calculate Inhibitor Concentration given Apparent Initial Enzyme Concentration, you need Initial Enzyme Concentration ([E₀]), Apparent Initial Enzyme Concentration (E0^app) & Enzyme Inhibitor Dissociation Constant (K_i). With our tool, you need to enter the respective value for Initial Enzyme Concentration, Apparent Initial Enzyme Concentration & Enzyme Inhibitor Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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