Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Calculator

✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Enzyme Inhibitor Dissociation Constant [K_i]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Final Rate Constant is the rate constant when the enzyme-substrate complex on reaction with inhibitor is converted into the enzyme catalyst and product.ⓘ Final Rate Constant [k₂]			+10% -10%

✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis [[E₀]]

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Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Solution

STEP 0: Pre-Calculation Summary

Formula Used

Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration)
[E₀] = (V₀*(K_M*(1+(I/K_i))+S))/(k₂*S)
This formula uses 7 Variables

Variables Used

Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Final Rate Constant - (Measured in 1 Per Second) - The Final Rate Constant is the rate constant when the enzyme-substrate complex on reaction with inhibitor is converted into the enzyme catalyst and product.

STEP 1: Convert Input(s) to Base Unit

Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Final Rate Constant: 23 1 Per Second --> 23 1 Per Second No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

[E₀] = (V₀*(K_M*(1+(I/K_i))+S))/(k₂*S) --> (450*(3000*(1+(9000/19000))+1500))/(23*1500)

Evaluating ... ...

[E₀] = 77.2311212814645

STEP 3: Convert Result to Output's Unit

77.2311212814645 Mole per Cubic Meter -->0.0772311212814645 Mole per Liter (Check conversion here)

FINAL ANSWER

0.0772311212814645 ≈ 0.077231 Mole per Liter <-- Initial Enzyme Concentration

(Calculation completed in 00.004 seconds)

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Created by Prashant Singh

K J Somaiya College of science (K J Somaiya), Mumbai

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< Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

LaTeX Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

LaTeX Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Formula

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What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?

Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis calculator uses Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration) to calculate the Initial Enzyme Concentration, The Initial enzyme concentration of competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Initial Enzyme Concentration is denoted by [E₀] symbol.

How to calculate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis using this online calculator? To use this online calculator for Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis, enter Initial Reaction Rate (V₀), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i), Substrate Concentration (S) & Final Rate Constant (k₂) and hit the calculate button. Here is how the Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis calculation can be explained with given input values -> 7.7E-5 = (450*(3000*(1+(9000/19000))+1500))/(23*1500).

FAQ

What is Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?

The Initial enzyme concentration of competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km and is represented as [E₀] = (V₀*(K_M*(1+(I/K_i))+S))/(k₂*S) or Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration). The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system, The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured, The Substrate Concentration is the number of moles of substrate per liter solution & The Final Rate Constant is the rate constant when the enzyme-substrate complex on reaction with inhibitor is converted into the enzyme catalyst and product.

How to calculate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?

The Initial enzyme concentration of competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km is calculated using Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration). To calculate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis, you need Initial Reaction Rate (V₀), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i), Substrate Concentration (S) & Final Rate Constant (k₂). With our tool, you need to enter the respective value for Initial Reaction Rate, Michaelis Constant, Inhibitor Concentration, Enzyme Inhibitor Dissociation Constant, Substrate Concentration & Final Rate Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Initial Enzyme Concentration?

In this formula, Initial Enzyme Concentration uses Initial Reaction Rate, Michaelis Constant, Inhibitor Concentration, Enzyme Inhibitor Dissociation Constant, Substrate Concentration & Final Rate Constant. We can use 1 other way(s) to calculate the same, which is/are as follows -

Initial Enzyme Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Substrate Concentration)

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