Forward Rate Constant given Reverse and Catalytic Rate Constant Solution

STEP 0: Pre-Calculation Summary
Formula Used
Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration))
kf = (kr+kcat)*(ES/(E*S))
This formula uses 6 Variables
Variables Used
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Catalyst Concentration - (Measured in Mole per Cubic Meter) - The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
STEP 1: Convert Input(s) to Base Unit
Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion ​here)
Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion ​here)
Catalyst Concentration: 25 Mole per Liter --> 25000 Mole per Cubic Meter (Check conversion ​here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion ​here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
kf = (kr+kcat)*(ES/(E*S)) --> (20000+0.65)*(10000/(25000*1500))
Evaluating ... ...
kf = 5.33350666666667
STEP 3: Convert Result to Output's Unit
5.33350666666667 1 Per Second --> No Conversion Required
FINAL ANSWER
5.33350666666667 5.333507 1 Per Second <-- Forward Rate Constant
(Calculation completed in 00.004 seconds)

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Rate Constants of Enzymatic Reaction Calculators

Forward Rate Constant in Enzymatic Reaction mechanism
​ LaTeX ​ Go Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Reverse Rate Constant in Enzymatic Reaction Mechanism
​ LaTeX ​ Go Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration
Catalytic Rate Constant at Low Substrate Concentration
​ LaTeX ​ Go Catalytic Rate Constant = (Initial Reaction Rate*Michaelis Constant)/(Initial Enzyme Concentration*Substrate Concentration)
Dissociation Rate Constant in Enzymatic Reaction Mechanism
​ LaTeX ​ Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant

Forward Rate Constant given Reverse and Catalytic Rate Constant Formula

​LaTeX ​Go
Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration))
kf = (kr+kcat)*(ES/(E*S))

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Forward Rate Constant given Reverse and Catalytic Rate Constant?

Forward Rate Constant given Reverse and Catalytic Rate Constant calculator uses Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration)) to calculate the Forward Rate Constant, The Forward rate constant given reverse and catalytic rate constant formula is defined as the relation with enzymatic catalyst and substrate concentration with an enzyme-substrate complex concentration in an enzymatic reaction mechanism. Forward Rate Constant is denoted by kf symbol.

How to calculate Forward Rate Constant given Reverse and Catalytic Rate Constant using this online calculator? To use this online calculator for Forward Rate Constant given Reverse and Catalytic Rate Constant, enter Reverse Rate Constant (kr), Catalytic Rate Constant (kcat), Enzyme Substrate Complex Concentration (ES), Catalyst Concentration (E) & Substrate Concentration (S) and hit the calculate button. Here is how the Forward Rate Constant given Reverse and Catalytic Rate Constant calculation can be explained with given input values -> 5.333507 = (20000+0.65)*(10000/(25000*1500)).

FAQ

What is Forward Rate Constant given Reverse and Catalytic Rate Constant?
The Forward rate constant given reverse and catalytic rate constant formula is defined as the relation with enzymatic catalyst and substrate concentration with an enzyme-substrate complex concentration in an enzymatic reaction mechanism and is represented as kf = (kr+kcat)*(ES/(E*S)) or Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration)). The Reverse Rate Constant is defined as the rate constant for the backward reaction, The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution & The Substrate Concentration is the number of moles of substrate per liter solution.
How to calculate Forward Rate Constant given Reverse and Catalytic Rate Constant?
The Forward rate constant given reverse and catalytic rate constant formula is defined as the relation with enzymatic catalyst and substrate concentration with an enzyme-substrate complex concentration in an enzymatic reaction mechanism is calculated using Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration)). To calculate Forward Rate Constant given Reverse and Catalytic Rate Constant, you need Reverse Rate Constant (kr), Catalytic Rate Constant (kcat), Enzyme Substrate Complex Concentration (ES), Catalyst Concentration (E) & Substrate Concentration (S). With our tool, you need to enter the respective value for Reverse Rate Constant, Catalytic Rate Constant, Enzyme Substrate Complex Concentration, Catalyst Concentration & Substrate Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Forward Rate Constant?
In this formula, Forward Rate Constant uses Reverse Rate Constant, Catalytic Rate Constant, Enzyme Substrate Complex Concentration, Catalyst Concentration & Substrate Concentration. We can use 2 other way(s) to calculate the same, which is/are as follows -
  • Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
  • Forward Rate Constant = (Reverse Rate Constant/Dissociation Rate Constant)
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