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Fluoroscence Intensity Calculator

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Fluoroscence and Phosphorescence Quantum Yield and Singlet Llifetime

✖Rate Constant of Fluoroscence is the rate at which spontaneous emission occurs.ⓘ Rate Constant of Fluoroscence [K_f]			+10% -10%
✖Absorption Intensity obtained by integrating the area under the absorption line—is proportional to the amount of the absorbing substance present.ⓘ Absorption Intensity [I_a]			+10% -10%
✖Rate Constant of Non Radiative Reaction is defined as the rate at which deactivation occurs in the form of heat energy.ⓘ Rate Constant of Non Radiative Reaction [K_NR]			+10% -10%

✖Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.ⓘ Fluoroscence Intensity [I_F]

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Fluoroscence Intensity Solution

STEP 0: Pre-Calculation Summary

Formula Used

Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)
I_F = (K_f*I_a)/(K_f+K_NR)
This formula uses 4 Variables

Variables Used

Fluorosence Intensity - (Measured in Watt per Square Meter) - Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.
Rate Constant of Fluoroscence - (Measured in Hertz) - Rate Constant of Fluoroscence is the rate at which spontaneous emission occurs.
Absorption Intensity - (Measured in Watt per Square Meter) - Absorption Intensity obtained by integrating the area under the absorption line—is proportional to the amount of the absorbing substance present.
Rate Constant of Non Radiative Reaction - (Measured in Hertz) - Rate Constant of Non Radiative Reaction is defined as the rate at which deactivation occurs in the form of heat energy.

STEP 1: Convert Input(s) to Base Unit

Rate Constant of Fluoroscence: 750 Revolution per Second --> 750 Hertz (Check conversion here)
Absorption Intensity: 250 Watt per Square Meter --> 250 Watt per Square Meter No Conversion Required
Rate Constant of Non Radiative Reaction: 35 Revolution per Second --> 35 Hertz (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

I_F = (K_f*I_a)/(K_f+K_NR) --> (750*250)/(750+35)

Evaluating ... ...

I_F = 238.853503184713

STEP 3: Convert Result to Output's Unit

238.853503184713 Watt per Square Meter --> No Conversion Required

FINAL ANSWER

238.853503184713 ≈ 238.8535 Watt per Square Meter <-- Fluorosence Intensity

(Calculation completed in 00.006 seconds)

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< Emission Spectroscopy Calculators

Degree of Exciplex Formation

LaTeX Go Degree of Exciplex Formation = (Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex)/(1+(Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex))

Collisional Energy Transfer

LaTeX Go Rate of Collisional Energy Transfer = Quenching Constant*Quencher Concentration given Degree of Exciplex*Singlet State Concentration

Difference in Acidity between Ground and Excited State

LaTeX Go Difference in pka = pKa of Excited State-pKa of Ground State

Equilibrium Constant for Exciplex Formation

LaTeX Go Equilibrium Constant for Coordinate Complexes = 1/(1-Degree of Exciplex Formation)-1

< Fluoroscence and Phosphorescence Calculators

Initial Intensity given Degree of Exciplex Formation

LaTeX Go Initial Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Fluoroscence Intensity without Quenching

LaTeX Go Intensity Without Quenching = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)

Fluoroscence Intensity

LaTeX Go Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Fluorescence Rate Constant

LaTeX Go Rate Constant of Fluoroscence = Rate of Fluoroscence/Singlet State Concentration

Fluoroscence Intensity Formula

LaTeX Go

Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)
I_F = (K_f*I_a)/(K_f+K_NR)

What is the difference between absorbance and fluorescence intensity?

Absorbance is measured as the difference in intensity between light passing through the reference and the sample, whereas fluorescence is measured directly without any reference beam. In other words, fluorescence is measured over a dark background, while absorbance is measured over a bright background.

What affects fluorescence intensity?

Three important factors influencing the intensity of fluorescence emission were theoretical analyzed, including the absorption ability of excitation photons, fluorescence quantum yield, and fluorescence saturation & fluorescence quenching.

How to Calculate Fluoroscence Intensity?

Fluoroscence Intensity calculator uses Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction) to calculate the Fluorosence Intensity, The Fluoroscence Intensity indicates how much light (photons) is emitted. It is the extent of emission and it depends on the concentration of the excited fluorophore. Fluorescence is created by the absorption of energy (light) by fluorescent molecules, called fluorophores. Fluorosence Intensity is denoted by I_F symbol.

How to calculate Fluoroscence Intensity using this online calculator? To use this online calculator for Fluoroscence Intensity, enter Rate Constant of Fluoroscence (K_f), Absorption Intensity (I_a) & Rate Constant of Non Radiative Reaction (K_NR) and hit the calculate button. Here is how the Fluoroscence Intensity calculation can be explained with given input values -> 238.8535 = (750*250)/(750+35).

FAQ

What is Fluoroscence Intensity?

The Fluoroscence Intensity indicates how much light (photons) is emitted. It is the extent of emission and it depends on the concentration of the excited fluorophore. Fluorescence is created by the absorption of energy (light) by fluorescent molecules, called fluorophores and is represented as I_F = (K_f*I_a)/(K_f+K_NR) or Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction). Rate Constant of Fluoroscence is the rate at which spontaneous emission occurs, Absorption Intensity obtained by integrating the area under the absorption line—is proportional to the amount of the absorbing substance present & Rate Constant of Non Radiative Reaction is defined as the rate at which deactivation occurs in the form of heat energy.

How to calculate Fluoroscence Intensity?

The Fluoroscence Intensity indicates how much light (photons) is emitted. It is the extent of emission and it depends on the concentration of the excited fluorophore. Fluorescence is created by the absorption of energy (light) by fluorescent molecules, called fluorophores is calculated using Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction). To calculate Fluoroscence Intensity, you need Rate Constant of Fluoroscence (K_f), Absorption Intensity (I_a) & Rate Constant of Non Radiative Reaction (K_NR). With our tool, you need to enter the respective value for Rate Constant of Fluoroscence, Absorption Intensity & Rate Constant of Non Radiative Reaction and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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