Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor Calculator

✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%

✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor [α^']

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Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration)
α^' = ((V_max*S)-(V₀*K_M))/(V₀*S)
This formula uses 5 Variables

Variables Used

Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

STEP 1: Convert Input(s) to Base Unit

Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

α^' = ((V_max*S)-(V₀*K_M))/(V₀*S) --> ((40000*1500)-(450*3000))/(450*1500)

Evaluating ... ...

α^' = 86.8888888888889

STEP 3: Convert Result to Output's Unit

86.8888888888889 --> No Conversion Required

FINAL ANSWER

86.8888888888889 ≈ 86.88889 <-- Enzyme Substrate Modifying Factor

(Calculation completed in 00.006 seconds)

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< Uncompetitive Inhibitor Calculators

Initial Reaction Rate in presence of Uncompetitive Inhibitor

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

LaTeX Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor Formula

LaTeX Go

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor?

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor calculator uses Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration) to calculate the Enzyme Substrate Modifying Factor, The Enzyme substrate modifying factor in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation. Enzyme Substrate Modifying Factor is denoted by α^' symbol.

How to calculate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor, enter Maximum Rate (V_max), Substrate Concentration (S), Initial Reaction Rate (V₀) & Michaelis Constant (K_M) and hit the calculate button. Here is how the Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 86.88889 = ((40000*1500)-(450*3000))/(450*1500).

FAQ

What is Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor?

The Enzyme substrate modifying factor in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation and is represented as α^' = ((V_max*S)-(V₀*K_M))/(V₀*S) or Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration). The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Substrate Concentration is the number of moles of substrate per liter solution, The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place & The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

How to calculate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor?

The Enzyme substrate modifying factor in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation is calculated using Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration). To calculate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor, you need Maximum Rate (V_max), Substrate Concentration (S), Initial Reaction Rate (V₀) & Michaelis Constant (K_M). With our tool, you need to enter the respective value for Maximum Rate, Substrate Concentration, Initial Reaction Rate & Michaelis Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Substrate Modifying Factor?

In this formula, Enzyme Substrate Modifying Factor uses Maximum Rate, Substrate Concentration, Initial Reaction Rate & Michaelis Constant. We can use 1 other way(s) to calculate the same, which is/are as follows -

Enzyme Substrate Modifying Factor = (1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant))

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