Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Calculator

✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.ⓘ Enzyme Substrate Inhibitor Complex Concentration [ESI]			+10% -10%

✖The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.ⓘ Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor [K_i']

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Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration
K_i' = (ES*I)/ESI
This formula uses 4 Variables

Variables Used

Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Inhibitor Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.

STEP 1: Convert Input(s) to Base Unit

Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Inhibitor Complex Concentration: 2.5 Mole per Liter --> 2500 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_i' = (ES*I)/ESI --> (10000*9000)/2500

Evaluating ... ...

K_i' = 36000

STEP 3: Convert Result to Output's Unit

36000 Mole per Cubic Meter -->36 Mole per Liter (Check conversion here)

FINAL ANSWER

36 Mole per Liter <-- Enzyme Substrate Dissociation Constant

(Calculation completed in 00.020 seconds)

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< Uncompetitive Inhibitor Calculators

Initial Reaction Rate in presence of Uncompetitive Inhibitor

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

LaTeX Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Formula

LaTeX Go

Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration
K_i' = (ES*I)/ESI

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor calculator uses Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration to calculate the Enzyme Substrate Dissociation Constant, The Enzyme substrate dissociation constant in presence of uncompetitive inhibitor formula is defined as related to the concentration of different enzyme species. Enzyme Substrate Dissociation Constant is denoted by K_i' symbol.

How to calculate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor, enter Enzyme Substrate Complex Concentration (ES), Inhibitor Concentration (I) & Enzyme Substrate Inhibitor Complex Concentration (ESI) and hit the calculate button. Here is how the Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 0.036 = (10000*9000)/2500.

FAQ

What is Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

The Enzyme substrate dissociation constant in presence of uncompetitive inhibitor formula is defined as related to the concentration of different enzyme species and is represented as K_i' = (ES*I)/ESI or Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration. The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.

How to calculate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

The Enzyme substrate dissociation constant in presence of uncompetitive inhibitor formula is defined as related to the concentration of different enzyme species is calculated using Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration. To calculate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor, you need Enzyme Substrate Complex Concentration (ES), Inhibitor Concentration (I) & Enzyme Substrate Inhibitor Complex Concentration (ESI). With our tool, you need to enter the respective value for Enzyme Substrate Complex Concentration, Inhibitor Concentration & Enzyme Substrate Inhibitor Complex Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Substrate Dissociation Constant?

In this formula, Enzyme Substrate Dissociation Constant uses Enzyme Substrate Complex Concentration, Inhibitor Concentration & Enzyme Substrate Inhibitor Complex Concentration. We can use 1 other way(s) to calculate the same, which is/are as follows -

Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

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