Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor Calculator

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Enzyme Substrate Modifying Factor [α^']			+10% -10%

✖The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.ⓘ Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor [K_i']

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Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)
K_i' = I/(α^'-1)
This formula uses 3 Variables

Variables Used

Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

STEP 1: Convert Input(s) to Base Unit

Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_i' = I/(α^'-1) --> 9000/(2-1)

Evaluating ... ...

K_i' = 9000

STEP 3: Convert Result to Output's Unit

9000 Mole per Cubic Meter -->9 Mole per Liter (Check conversion here)

FINAL ANSWER

9 Mole per Liter <-- Enzyme Substrate Dissociation Constant

(Calculation completed in 00.004 seconds)

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< Uncompetitive Inhibitor Calculators

Initial Reaction Rate in presence of Uncompetitive Inhibitor

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

LaTeX Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor Formula

LaTeX Go

Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)
K_i' = I/(α^'-1)

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor?

Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor calculator uses Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1) to calculate the Enzyme Substrate Dissociation Constant, The Enzyme substrate dissociation constant given enzyme substrate modifying factor formula is defined as the relation with inhibitor concentration and with the enzyme-substrate modifying factor. Enzyme Substrate Dissociation Constant is denoted by K_i' symbol.

How to calculate Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor using this online calculator? To use this online calculator for Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor, enter Inhibitor Concentration (I) & Enzyme Substrate Modifying Factor (α^') and hit the calculate button. Here is how the Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor calculation can be explained with given input values -> 0.009 = 9000/(2-1).

FAQ

What is Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor?

The Enzyme substrate dissociation constant given enzyme substrate modifying factor formula is defined as the relation with inhibitor concentration and with the enzyme-substrate modifying factor and is represented as K_i' = I/(α^'-1) or Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

How to calculate Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor?

The Enzyme substrate dissociation constant given enzyme substrate modifying factor formula is defined as the relation with inhibitor concentration and with the enzyme-substrate modifying factor is calculated using Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1). To calculate Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor, you need Inhibitor Concentration (I) & Enzyme Substrate Modifying Factor (α^'). With our tool, you need to enter the respective value for Inhibitor Concentration & Enzyme Substrate Modifying Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Substrate Dissociation Constant?

In this formula, Enzyme Substrate Dissociation Constant uses Inhibitor Concentration & Enzyme Substrate Modifying Factor. We can use 1 other way(s) to calculate the same, which is/are as follows -

Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration

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