Enzyme Substrate Complex Concentration given Dissociation Rate Constant Solution

STEP 0: Pre-Calculation Summary
Formula Used
Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)
ES = ([E0]*S)/(KD+S)
This formula uses 4 Variables
Variables Used
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Dissociation Rate Constant - (Measured in Mole per Cubic Meter) - The Dissociation Rate Constant is the ratio of reverse and forward rate constant.
STEP 1: Convert Input(s) to Base Unit
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion ​here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion ​here)
Dissociation Rate Constant: 5.7 Mole per Liter --> 5700 Mole per Cubic Meter (Check conversion ​here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
ES = ([E0]*S)/(KD+S) --> (100000*1500)/(5700+1500)
Evaluating ... ...
ES = 20833.3333333333
STEP 3: Convert Result to Output's Unit
20833.3333333333 Mole per Cubic Meter -->20.8333333333333 Mole per Liter (Check conversion ​here)
FINAL ANSWER
20.8333333333333 20.83333 Mole per Liter <-- Enzyme Substrate Complex Concentration
(Calculation completed in 00.020 seconds)

Credits

Creator Image
Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
Prashant Singh has created this Calculator and 700+ more calculators!
Verifier Image
Verified by Prerana Bakli
University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA
Prerana Bakli has verified this Calculator and 1600+ more calculators!

Complex Concentration Calculators

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration
​ LaTeX ​ Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration
​ LaTeX ​ Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Initial Enzyme Concentration at Low Substrate Concentration
​ LaTeX ​ Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)
Substrate Concentration given Maximum Rate at Low Concentration
​ LaTeX ​ Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Enzyme Substrate Complex Concentration given Dissociation Rate Constant Formula

​LaTeX ​Go
Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)
ES = ([E0]*S)/(KD+S)

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant?

Enzyme Substrate Complex Concentration given Dissociation Rate Constant calculator uses Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration) to calculate the Enzyme Substrate Complex Concentration, The Enzyme substrate complex concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant using this online calculator? To use this online calculator for Enzyme Substrate Complex Concentration given Dissociation Rate Constant, enter Initial Enzyme Concentration ([E0]), Substrate Concentration (S) & Dissociation Rate Constant (KD) and hit the calculate button. Here is how the Enzyme Substrate Complex Concentration given Dissociation Rate Constant calculation can be explained with given input values -> 0.020833 = (100000*1500)/(5700+1500).

FAQ

What is Enzyme Substrate Complex Concentration given Dissociation Rate Constant?
The Enzyme substrate complex concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate and is represented as ES = ([E0]*S)/(KD+S) or Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration). The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Substrate Concentration is the number of moles of substrate per liter solution & The Dissociation Rate Constant is the ratio of reverse and forward rate constant.
How to calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant?
The Enzyme substrate complex concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate is calculated using Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration). To calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant, you need Initial Enzyme Concentration ([E0]), Substrate Concentration (S) & Dissociation Rate Constant (KD). With our tool, you need to enter the respective value for Initial Enzyme Concentration, Substrate Concentration & Dissociation Rate Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Enzyme Substrate Complex Concentration?
In this formula, Enzyme Substrate Complex Concentration uses Initial Enzyme Concentration, Substrate Concentration & Dissociation Rate Constant. We can use 1 other way(s) to calculate the same, which is/are as follows -
  • Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))
Let Others Know
Facebook
Twitter
Reddit
LinkedIn
Email
WhatsApp
Copied!