Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Calculator

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Enzyme Inhibitor Dissociation Constant [K_i]			+10% -10%

✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis [ES]

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Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)
ES = (S*[E₀])/(K_M*(1+(I/K_i))+S)
This formula uses 6 Variables

Variables Used

Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

STEP 1: Convert Input(s) to Base Unit

Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

ES = (S*[E₀])/(K_M*(1+(I/K_i))+S) --> (1500*100000)/(3000*(1+(9000/19000))+1500)

Evaluating ... ...

ES = 25333.3333333333

STEP 3: Convert Result to Output's Unit

25333.3333333333 Mole per Cubic Meter -->25.3333333333333 Mole per Liter (Check conversion here)

FINAL ANSWER

25.3333333333333 ≈ 25.33333 Mole per Liter <-- Enzyme Substrate Complex Concentration

(Calculation completed in 00.076 seconds)

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< Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

LaTeX Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

LaTeX Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

< Important Formulas on Enzyme Kinetics Calculators

Initial Reaction Rate given Dissociation Rate Constant

LaTeX Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

LaTeX Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Modifying Factor of Enzyme Substrate Complex

LaTeX Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

LaTeX Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Formula

LaTeX Go

What is enzyme catalysis?

Enzyme catalysis is the increase in the rate of a process by a biological molecule, an "enzyme". Most enzymes are proteins, and most such processes are chemical reactions. Within the enzyme, generally catalysis occurs at a localized site, called the active site.

How to Calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis calculator uses Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration) to calculate the Enzyme Substrate Complex Concentration, The Enzyme substrate complex concentration for competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate which can then be used to determine values such as Vmax, initial velocity, and Km. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis using this online calculator? To use this online calculator for Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis, enter Substrate Concentration (S), Initial Enzyme Concentration ([E₀]), Michaelis Constant (K_M), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i) and hit the calculate button. Here is how the Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis calculation can be explained with given input values -> 0.025333 = (1500*100000)/(3000*(1+(9000/19000))+1500).

FAQ

What is Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

The Enzyme substrate complex concentration for competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate which can then be used to determine values such as Vmax, initial velocity, and Km and is represented as ES = (S*[E₀])/(K_M*(1+(I/K_i))+S) or Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration). The Substrate Concentration is the number of moles of substrate per liter solution, The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

How to calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

The Enzyme substrate complex concentration for competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate which can then be used to determine values such as Vmax, initial velocity, and Km is calculated using Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration). To calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis, you need Substrate Concentration (S), Initial Enzyme Concentration ([E₀]), Michaelis Constant (K_M), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i). With our tool, you need to enter the respective value for Substrate Concentration, Initial Enzyme Concentration, Michaelis Constant, Inhibitor Concentration & Enzyme Inhibitor Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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