Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor Calculator

✖The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.ⓘ Enzyme Substrate Dissociation Constant [K_i']			+10% -10%
✖The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.ⓘ Enzyme Substrate Inhibitor Complex Concentration [ESI]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%

✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor [ES]

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Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration
ES = (K_i'*ESI)/I
This formula uses 4 Variables

Variables Used

Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.
Enzyme Substrate Inhibitor Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

STEP 1: Convert Input(s) to Base Unit

Enzyme Substrate Dissociation Constant: 15 Mole per Liter --> 15000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Inhibitor Complex Concentration: 2.5 Mole per Liter --> 2500 Mole per Cubic Meter (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

ES = (K_i'*ESI)/I --> (15000*2500)/9000

Evaluating ... ...

ES = 4166.66666666667

STEP 3: Convert Result to Output's Unit

4166.66666666667 Mole per Cubic Meter -->4.16666666666667 Mole per Liter (Check conversion here)

FINAL ANSWER

4.16666666666667 ≈ 4.166667 Mole per Liter <-- Enzyme Substrate Complex Concentration

(Calculation completed in 00.006 seconds)

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< Uncompetitive Inhibitor Calculators

Initial Reaction Rate in presence of Uncompetitive Inhibitor

LaTeX Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

LaTeX Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

LaTeX Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

LaTeX Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor Formula

LaTeX Go

Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration
ES = (K_i'*ESI)/I

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor?

Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor calculator uses Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration to calculate the Enzyme Substrate Complex Concentration, The Enzyme substrate complex concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different species of enzyme species. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor, enter Enzyme Substrate Dissociation Constant (K_i'), Enzyme Substrate Inhibitor Complex Concentration (ESI) & Inhibitor Concentration (I) and hit the calculate button. Here is how the Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 0.004167 = (15000*2500)/9000.

FAQ

What is Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor?

The Enzyme substrate complex concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different species of enzyme species and is represented as ES = (K_i'*ESI)/I or Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration. The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product, The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex & The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

How to calculate Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor?

The Enzyme substrate complex concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different species of enzyme species is calculated using Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration. To calculate Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor, you need Enzyme Substrate Dissociation Constant (K_i'), Enzyme Substrate Inhibitor Complex Concentration (ESI) & Inhibitor Concentration (I). With our tool, you need to enter the respective value for Enzyme Substrate Dissociation Constant, Enzyme Substrate Inhibitor Complex Concentration & Inhibitor Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Substrate Complex Concentration?

In this formula, Enzyme Substrate Complex Concentration uses Enzyme Substrate Dissociation Constant, Enzyme Substrate Inhibitor Complex Concentration & Inhibitor Concentration. We can use 1 other way(s) to calculate the same, which is/are as follows -

Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)

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