What is competitive Inhibition?
In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.
How to Calculate Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate?
Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate calculator uses Enzyme Inhibitor Complex Concentration = (Initial Reaction Rate/Final Rate Constant) to calculate the Enzyme Inhibitor Complex Concentration, The Enzyme substrate complex concentration given rate constant and initial rate formula is defined as the ratio of the initial rate of the system to the rate constant of the reaction converting enzyme-substrate intermediate to the product. Enzyme Inhibitor Complex Concentration is denoted by EI symbol.
How to calculate Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate using this online calculator? To use this online calculator for Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate, enter Initial Reaction Rate (V0) & Final Rate Constant (k2) and hit the calculate button. Here is how the Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate calculation can be explained with given input values -> 2E-5 = (450/23).