Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Solution

STEP 0: Pre-Calculation Summary
Formula Used
Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)
ES = (S*[E0])/(KM*(1+(I/Ki))+S)
This formula uses 6 Variables
Variables Used
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
STEP 1: Convert Input(s) to Base Unit
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion ​here)
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion ​here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion ​here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion ​here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion ​here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
ES = (S*[E0])/(KM*(1+(I/Ki))+S) --> (1500*100000)/(3000*(1+(9000/19000))+1500)
Evaluating ... ...
ES = 25333.3333333333
STEP 3: Convert Result to Output's Unit
25333.3333333333 Mole per Cubic Meter -->25.3333333333333 Mole per Liter (Check conversion ​here)
FINAL ANSWER
25.3333333333333 25.33333 Mole per Liter <-- Enzyme Substrate Complex Concentration
(Calculation completed in 00.020 seconds)

Credits

Creator Image
Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
Prashant Singh has created this Calculator and 700+ more calculators!
Verifier Image
Verified by Prerana Bakli
University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA
Prerana Bakli has verified this Calculator and 1600+ more calculators!

Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis
​ LaTeX ​ Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration
​ LaTeX ​ Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)
Substrate Concentration in Competitive Inhibition given Maximum Rate of System
​ LaTeX ​ Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)
Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition
​ LaTeX ​ Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

Important Formulas on Enzyme Kinetics Calculators

Initial Reaction Rate given Dissociation Rate Constant
​ LaTeX ​ Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)
Maximum Rate given Dissociation Rate Constant
​ LaTeX ​ Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration
Modifying Factor of Enzyme Substrate Complex
​ LaTeX ​ Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)
Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration
​ LaTeX ​ Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Formula

​LaTeX ​Go
Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)
ES = (S*[E0])/(KM*(1+(I/Ki))+S)

What is enzyme catalysis?

Enzyme catalysis is the increase in the rate of a process by a biological molecule, an "enzyme". Most enzymes are proteins, and most such processes are chemical reactions. Within the enzyme, generally catalysis occurs at a localized site, called the active site.

How to Calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis calculator uses Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration) to calculate the Enzyme Substrate Complex Concentration, The Enzyme substrate complex concentration for competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate which can then be used to determine values such as Vmax, initial velocity, and Km. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis using this online calculator? To use this online calculator for Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis, enter Substrate Concentration (S), Initial Enzyme Concentration ([E0]), Michaelis Constant (KM), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (Ki) and hit the calculate button. Here is how the Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis calculation can be explained with given input values -> 0.025333 = (1500*100000)/(3000*(1+(9000/19000))+1500).

FAQ

What is Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?
The Enzyme substrate complex concentration for competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate which can then be used to determine values such as Vmax, initial velocity, and Km and is represented as ES = (S*[E0])/(KM*(1+(I/Ki))+S) or Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration). The Substrate Concentration is the number of moles of substrate per liter solution, The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
How to calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?
The Enzyme substrate complex concentration for competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate which can then be used to determine values such as Vmax, initial velocity, and Km is calculated using Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration). To calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis, you need Substrate Concentration (S), Initial Enzyme Concentration ([E0]), Michaelis Constant (KM), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (Ki). With our tool, you need to enter the respective value for Substrate Concentration, Initial Enzyme Concentration, Michaelis Constant, Inhibitor Concentration & Enzyme Inhibitor Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
Let Others Know
Facebook
Twitter
Reddit
LinkedIn
Email
WhatsApp
Copied!