Efficiency of Energy Transfer using Photobleaching Decay Time Constant Calculator

✖The Photobleaching Decay Time Constant is the photobleaching decay time constant of the donor in the absence of the acceptor.ⓘ Photobleaching Decay Time Constant [ζ_pb]			+10% -10%
✖The Photobleaching Decay Time Constant with FRET is the photobleaching decay time constant of the donor in the presence of the acceptor.ⓘ Photobleaching Decay Time Constant with FRET [ζ_pbA]			+10% -10%

✖The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).ⓘ Efficiency of Energy Transfer using Photobleaching Decay Time Constant [E]

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Efficiency of Energy Transfer using Photobleaching Decay Time Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)
E = 1-(ζ_pb/ζ_pbA)
This formula uses 3 Variables

Variables Used

Efficiency of Energy Transfer - The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).
Photobleaching Decay Time Constant - The Photobleaching Decay Time Constant is the photobleaching decay time constant of the donor in the absence of the acceptor.
Photobleaching Decay Time Constant with FRET - The Photobleaching Decay Time Constant with FRET is the photobleaching decay time constant of the donor in the presence of the acceptor.

STEP 1: Convert Input(s) to Base Unit

Photobleaching Decay Time Constant: 0.002 --> No Conversion Required
Photobleaching Decay Time Constant with FRET: 0.003 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

E = 1-(ζ_pb/ζ_pbA) --> 1-(0.002/0.003)

Evaluating ... ...

E = 0.333333333333333

STEP 3: Convert Result to Output's Unit

0.333333333333333 --> No Conversion Required

FINAL ANSWER

0.333333333333333 ≈ 0.333333 <-- Efficiency of Energy Transfer

(Calculation completed in 00.004 seconds)

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< Förster resonance energy transfer Calculators

Forster Critical Distance

LaTeX Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)

Efficiency of Energy Transfer using Rate of Energy Transfer

LaTeX Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)

Efficiency of Energy Transfer using Distances

LaTeX Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime

LaTeX Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

Efficiency of Energy Transfer using Photobleaching Decay Time Constant Formula

LaTeX Go

Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)
E = 1-(ζ_pb/ζ_pbA)

What is photobleaching?

In optics, photobleaching is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between the fluorophore and surrounding molecules.

How to Calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant?

Efficiency of Energy Transfer using Photobleaching Decay Time Constant calculator uses Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET) to calculate the Efficiency of Energy Transfer, The Efficiency of Energy Transfer using Photobleaching Decay Time Constant formula is defined as the ratio of the difference of photobleaching decay time constant in presence and absence of acceptor respectively, to the photobleaching decay time constant in the presence of acceptor. Efficiency of Energy Transfer is denoted by E symbol.

How to calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant using this online calculator? To use this online calculator for Efficiency of Energy Transfer using Photobleaching Decay Time Constant, enter Photobleaching Decay Time Constant (ζ_pb) & Photobleaching Decay Time Constant with FRET (ζ_pbA) and hit the calculate button. Here is how the Efficiency of Energy Transfer using Photobleaching Decay Time Constant calculation can be explained with given input values -> 0.333333 = 1-(0.002/0.003).

FAQ

What is Efficiency of Energy Transfer using Photobleaching Decay Time Constant?

The Efficiency of Energy Transfer using Photobleaching Decay Time Constant formula is defined as the ratio of the difference of photobleaching decay time constant in presence and absence of acceptor respectively, to the photobleaching decay time constant in the presence of acceptor and is represented as E = 1-(ζ_pb/ζ_pbA) or Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET). The Photobleaching Decay Time Constant is the photobleaching decay time constant of the donor in the absence of the acceptor & The Photobleaching Decay Time Constant with FRET is the photobleaching decay time constant of the donor in the presence of the acceptor.

How to calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant?

The Efficiency of Energy Transfer using Photobleaching Decay Time Constant formula is defined as the ratio of the difference of photobleaching decay time constant in presence and absence of acceptor respectively, to the photobleaching decay time constant in the presence of acceptor is calculated using Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET). To calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant, you need Photobleaching Decay Time Constant (ζ_pb) & Photobleaching Decay Time Constant with FRET (ζ_pbA). With our tool, you need to enter the respective value for Photobleaching Decay Time Constant & Photobleaching Decay Time Constant with FRET and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Efficiency of Energy Transfer?

In this formula, Efficiency of Energy Transfer uses Photobleaching Decay Time Constant & Photobleaching Decay Time Constant with FRET. We can use 3 other way(s) to calculate the same, which is/are as follows -

Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)
Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)
Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

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