Dissociation Rate Constant given Concentration of Enzyme and Substrate Solution

STEP 0: Pre-Calculation Summary
Formula Used
Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration
KD = ((Vmax*S)/V0)-S
This formula uses 4 Variables
Variables Used
Dissociation Rate Constant - (Measured in Mole per Cubic Meter) - The Dissociation Rate Constant is the ratio of reverse and forward rate constant.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
STEP 1: Convert Input(s) to Base Unit
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion ​here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion ​here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion ​here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
KD = ((Vmax*S)/V0)-S --> ((40000*1500)/450)-1500
Evaluating ... ...
KD = 131833.333333333
STEP 3: Convert Result to Output's Unit
131833.333333333 Mole per Cubic Meter -->131.833333333333 Mole per Liter (Check conversion ​here)
FINAL ANSWER
131.833333333333 131.8333 Mole per Liter <-- Dissociation Rate Constant
(Calculation completed in 00.004 seconds)

Credits

Creator Image
Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
Prashant Singh has created this Calculator and 700+ more calculators!
Verifier Image
Verified by Prerana Bakli
University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA
Prerana Bakli has verified this Calculator and 1600+ more calculators!

Rate Constants of Enzymatic Reaction Calculators

Forward Rate Constant in Enzymatic Reaction mechanism
​ LaTeX ​ Go Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Reverse Rate Constant in Enzymatic Reaction Mechanism
​ LaTeX ​ Go Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration
Catalytic Rate Constant at Low Substrate Concentration
​ LaTeX ​ Go Catalytic Rate Constant = (Initial Reaction Rate*Michaelis Constant)/(Initial Enzyme Concentration*Substrate Concentration)
Dissociation Rate Constant in Enzymatic Reaction Mechanism
​ LaTeX ​ Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant

Dissociation Rate Constant given Concentration of Enzyme and Substrate Formula

​LaTeX ​Go
Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration
KD = ((Vmax*S)/V0)-S

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Dissociation Rate Constant given Concentration of Enzyme and Substrate?

Dissociation Rate Constant given Concentration of Enzyme and Substrate calculator uses Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration to calculate the Dissociation Rate Constant, The Dissociation rate constant given concentration of enzyme and substrate formula is defined as the relation with the maximum rate achieved by the system, initial rate of the system, and with substrate concentration. Dissociation Rate Constant is denoted by KD symbol.

How to calculate Dissociation Rate Constant given Concentration of Enzyme and Substrate using this online calculator? To use this online calculator for Dissociation Rate Constant given Concentration of Enzyme and Substrate, enter Maximum Rate (Vmax), Substrate Concentration (S) & Initial Reaction Rate (V0) and hit the calculate button. Here is how the Dissociation Rate Constant given Concentration of Enzyme and Substrate calculation can be explained with given input values -> 0.131833 = ((40000*1500)/450)-1500.

FAQ

What is Dissociation Rate Constant given Concentration of Enzyme and Substrate?
The Dissociation rate constant given concentration of enzyme and substrate formula is defined as the relation with the maximum rate achieved by the system, initial rate of the system, and with substrate concentration and is represented as KD = ((Vmax*S)/V0)-S or Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration. The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Substrate Concentration is the number of moles of substrate per liter solution & The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
How to calculate Dissociation Rate Constant given Concentration of Enzyme and Substrate?
The Dissociation rate constant given concentration of enzyme and substrate formula is defined as the relation with the maximum rate achieved by the system, initial rate of the system, and with substrate concentration is calculated using Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration. To calculate Dissociation Rate Constant given Concentration of Enzyme and Substrate, you need Maximum Rate (Vmax), Substrate Concentration (S) & Initial Reaction Rate (V0). With our tool, you need to enter the respective value for Maximum Rate, Substrate Concentration & Initial Reaction Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Dissociation Rate Constant?
In this formula, Dissociation Rate Constant uses Maximum Rate, Substrate Concentration & Initial Reaction Rate. We can use 2 other way(s) to calculate the same, which is/are as follows -
  • Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant
  • Dissociation Rate Constant = ((Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration
Let Others Know
Facebook
Twitter
Reddit
LinkedIn
Email
WhatsApp
Copied!