Dissociation Constant of Enzyme given Modifying Factor of Enzyme Calculator

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.ⓘ Enzyme Modifying Factor [α]			+10% -10%

✖Enzyme Inhibitor Dissociation Constant given MF is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Dissociation Constant of Enzyme given Modifying Factor of Enzyme [K_ei]

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Dissociation Constant of Enzyme given Modifying Factor of Enzyme Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)
K_ei = I/(α-1)
This formula uses 3 Variables

Variables Used

Enzyme Inhibitor Dissociation Constant given MF - (Measured in Mole per Cubic Meter) - Enzyme Inhibitor Dissociation Constant given MF is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Modifying Factor - The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.

STEP 1: Convert Input(s) to Base Unit

Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Modifying Factor: 5 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_ei = I/(α-1) --> 9000/(5-1)

Evaluating ... ...

K_ei = 2250

STEP 3: Convert Result to Output's Unit

2250 Mole per Cubic Meter -->2.25 Mole per Liter (Check conversion here)

FINAL ANSWER

2.25 Mole per Liter <-- Enzyme Inhibitor Dissociation Constant given MF

(Calculation completed in 00.004 seconds)

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< Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

LaTeX Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

LaTeX Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

< Important Formulas on Enzyme Kinetics Calculators

Initial Reaction Rate given Dissociation Rate Constant

LaTeX Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

LaTeX Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Modifying Factor of Enzyme Substrate Complex

LaTeX Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

LaTeX Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Dissociation Constant of Enzyme given Modifying Factor of Enzyme Formula

LaTeX Go

Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)
K_ei = I/(α-1)

What is dissociation constant?

The dissociation constant (Kd) quantifies the equilibrium between a ligand (L) being free in solution and bound to a site in a protein (EL). It corresponds to the affinity which the ligand has for the binding site. Ligands with higher, more favorable free energy of association bind “tighter” and therefore have greater preference for the bound state. Because Kd is defined as a dissociation constant, higher affinity ligands have lower Kd values.

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Dissociation Constant of Enzyme given Modifying Factor of Enzyme?

Dissociation Constant of Enzyme given Modifying Factor of Enzyme calculator uses Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1) to calculate the Enzyme Inhibitor Dissociation Constant given MF, The Dissociation constant of enzyme given modifying factor of enzyme formula is defined as the relation with the inhibitor concentration and with the modifying factor of the enzyme. Enzyme Inhibitor Dissociation Constant given MF is denoted by K_ei symbol.

How to calculate Dissociation Constant of Enzyme given Modifying Factor of Enzyme using this online calculator? To use this online calculator for Dissociation Constant of Enzyme given Modifying Factor of Enzyme, enter Inhibitor Concentration (I) & Enzyme Modifying Factor (α) and hit the calculate button. Here is how the Dissociation Constant of Enzyme given Modifying Factor of Enzyme calculation can be explained with given input values -> 2250 = 9000/(5-1).

FAQ

What is Dissociation Constant of Enzyme given Modifying Factor of Enzyme?

The Dissociation constant of enzyme given modifying factor of enzyme formula is defined as the relation with the inhibitor concentration and with the modifying factor of the enzyme and is represented as K_ei = I/(α-1) or Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.

How to calculate Dissociation Constant of Enzyme given Modifying Factor of Enzyme?

The Dissociation constant of enzyme given modifying factor of enzyme formula is defined as the relation with the inhibitor concentration and with the modifying factor of the enzyme is calculated using Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1). To calculate Dissociation Constant of Enzyme given Modifying Factor of Enzyme, you need Inhibitor Concentration (I) & Enzyme Modifying Factor (α). With our tool, you need to enter the respective value for Inhibitor Concentration & Enzyme Modifying Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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