What is competitive Inhibition?
In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.
How to Calculate Dissociation Constant given Enzyme Substrate Complex Concentration?
Dissociation Constant given Enzyme Substrate Complex Concentration calculator uses Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1) to calculate the Enzyme Inhibitor Dissociation Constant, The Dissociation constant given enzyme substrate complex concentrationformula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Enzyme Inhibitor Dissociation Constant is denoted by Ki symbol.
How to calculate Dissociation Constant given Enzyme Substrate Complex Concentration using this online calculator? To use this online calculator for Dissociation Constant given Enzyme Substrate Complex Concentration, enter Inhibitor Concentration (I), Initial Enzyme Concentration ([E0]), Substrate Concentration (S), Enzyme Substrate Complex Concentration (ES) & Michaelis Constant (KM) and hit the calculate button. Here is how the Dissociation Constant given Enzyme Substrate Complex Concentration calculation can be explained with given input values -> 0.002571 = 9000/(((((100000*1500)/10000)-1500)/3000)-1).