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Concentration of Unbound Ligand Calculator

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✖Total Bound Ligand of Complex is the concentration of the ligand that is involved in complexation.ⓘ Total Bound Ligand of Complex [T_CL°]			+10% -10%
✖Formation Factor for Complexation is the ratio of the total concentration of the ligand bound to the metal ion to the total concentration of the metal ion.ⓘ Formation Factor for Complexation [n_factor]			+10% -10%
✖Total Metal Concentration of complex is the concentration of the metal ion involved in complexation.ⓘ Total Metal Concentration of Complex [T_CM°]			+10% -10%

✖Concentration of Unbound Ligand is the concentration of the ligands that do not form complex to the metal ion.ⓘ Concentration of Unbound Ligand [L_complex]

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Concentration of Unbound Ligand Solution

STEP 0: Pre-Calculation Summary

Formula Used

Concentration of Unbound Ligand = Total Bound Ligand of Complex-(Formation Factor for Complexation*Total Metal Concentration of Complex)
L_complex = T_CL°-(n_factor*T_CM°)
This formula uses 4 Variables

Variables Used

Concentration of Unbound Ligand - (Measured in Mole per Cubic Meter) - Concentration of Unbound Ligand is the concentration of the ligands that do not form complex to the metal ion.
Total Bound Ligand of Complex - (Measured in Mole per Cubic Meter) - Total Bound Ligand of Complex is the concentration of the ligand that is involved in complexation.
Formation Factor for Complexation - Formation Factor for Complexation is the ratio of the total concentration of the ligand bound to the metal ion to the total concentration of the metal ion.
Total Metal Concentration of Complex - (Measured in Mole per Cubic Meter) - Total Metal Concentration of complex is the concentration of the metal ion involved in complexation.

STEP 1: Convert Input(s) to Base Unit

Total Bound Ligand of Complex: 0.25 Mole per Liter --> 250 Mole per Cubic Meter (Check conversion here)
Formation Factor for Complexation: 0.5 --> No Conversion Required
Total Metal Concentration of Complex: 0.09 Mole per Liter --> 90 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

L_complex = T_CL°-(n_factor*T_CM°) --> 250-(0.5*90)

Evaluating ... ...

L_complex = 205

STEP 3: Convert Result to Output's Unit

205 Mole per Cubic Meter -->0.205 Mole per Liter (Check conversion here)

FINAL ANSWER

0.205 Mole per Liter <-- Concentration of Unbound Ligand

(Calculation completed in 00.004 seconds)

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< Complex Equilibria Calculators

Change in Stability Constant

LaTeX Go Change in Stability Constant of the Complex = 10^(log10(Stability Constant for Ternary Complex)-(log10(Stability Constant for Binary Complex MA)+log10(Stability Constant for Binary Complex MB)))

Stability Constant of Ternary Complexes

LaTeX Go Stability Constant for Ternary Complex = (Concentration of Ternary Complex)/(Concentration of Binary Ion*Ligand Concentration of the Complex)

Stability Constant of Binary Complexes

LaTeX Go Stability Constant for Binary Complex = (Concentration of Binary Ion)/(Concentration of Metal in Complex*Ligand Concentration of the Complex)

Stability Constant of Complex Compound

LaTeX Go Stability Constant of Complex = 1/Dissociation Constant of the Complex

Concentration of Unbound Ligand Formula

LaTeX Go

Concentration of Unbound Ligand = Total Bound Ligand of Complex-(Formation Factor for Complexation*Total Metal Concentration of Complex)
L_complex = T_CL°-(n_factor*T_CM°)

What is Ligand binding affinity ?

The binding of a ligand to a single binding site is definable by the concentration of the binding site (Bmax) and the concentration of unbound ligand at which the binding site is 50% occupied (Kd). The Kd is also known as the equilibrium dissociation constant. Its reciprocal is a measure of affinity.

How to Calculate Concentration of Unbound Ligand?

Concentration of Unbound Ligand calculator uses Concentration of Unbound Ligand = Total Bound Ligand of Complex-(Formation Factor for Complexation*Total Metal Concentration of Complex) to calculate the Concentration of Unbound Ligand, The Concentration of Unbound Ligand is defined as the concentration of ligand which is not forming complex with the metal ion. Concentration of Unbound Ligand is denoted by L_complex symbol.

How to calculate Concentration of Unbound Ligand using this online calculator? To use this online calculator for Concentration of Unbound Ligand, enter Total Bound Ligand of Complex (T_CL°), Formation Factor for Complexation (n_factor) & Total Metal Concentration of Complex (T_CM°) and hit the calculate button. Here is how the Concentration of Unbound Ligand calculation can be explained with given input values -> 0.000205 = 250-(0.5*90).

FAQ

What is Concentration of Unbound Ligand?

The Concentration of Unbound Ligand is defined as the concentration of ligand which is not forming complex with the metal ion and is represented as L_complex = T_CL°-(n_factor*T_CM°) or Concentration of Unbound Ligand = Total Bound Ligand of Complex-(Formation Factor for Complexation*Total Metal Concentration of Complex). Total Bound Ligand of Complex is the concentration of the ligand that is involved in complexation, Formation Factor for Complexation is the ratio of the total concentration of the ligand bound to the metal ion to the total concentration of the metal ion & Total Metal Concentration of complex is the concentration of the metal ion involved in complexation.

How to calculate Concentration of Unbound Ligand?

The Concentration of Unbound Ligand is defined as the concentration of ligand which is not forming complex with the metal ion is calculated using Concentration of Unbound Ligand = Total Bound Ligand of Complex-(Formation Factor for Complexation*Total Metal Concentration of Complex). To calculate Concentration of Unbound Ligand, you need Total Bound Ligand of Complex (T_CL°), Formation Factor for Complexation (n_factor) & Total Metal Concentration of Complex (T_CM°). With our tool, you need to enter the respective value for Total Bound Ligand of Complex, Formation Factor for Complexation & Total Metal Concentration of Complex and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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