What is competitive Inhibition?
In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.
How to Calculate Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law?
Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law calculator uses Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration-Catalyst Concentration-Enzyme Inhibitor Complex Concentration) to calculate the Enzyme Substrate Complex Concentration, The Concentration of enzyme substrate complex in presence of inhibitor by enzyme conservation law formula is defined as the relation with the concentration of each of the enzyme species of the system. Enzyme Substrate Complex Concentration is denoted by ES symbol.
How to calculate Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law using this online calculator? To use this online calculator for Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law, enter Initial Enzyme Concentration ([E0]), Catalyst Concentration (E) & Enzyme Inhibitor Complex Concentration (EI) and hit the calculate button. Here is how the Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law calculation can be explained with given input values -> 0.046 = (100000-25000-29000).