Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law Solution

STEP 0: Pre-Calculation Summary
Formula Used
Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration-Catalyst Concentration-Enzyme Inhibitor Complex Concentration)
ES = ([E0]-E-EI)
This formula uses 4 Variables
Variables Used
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Catalyst Concentration - (Measured in Mole per Cubic Meter) - The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.
Enzyme Inhibitor Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Complex Concentration is the number of moles of enzyme inhibitor substrate complex per liter of the enzymatic solution.
STEP 1: Convert Input(s) to Base Unit
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion ​here)
Catalyst Concentration: 25 Mole per Liter --> 25000 Mole per Cubic Meter (Check conversion ​here)
Enzyme Inhibitor Complex Concentration: 29 Mole per Liter --> 29000 Mole per Cubic Meter (Check conversion ​here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
ES = ([E0]-E-EI) --> (100000-25000-29000)
Evaluating ... ...
ES = 46000
STEP 3: Convert Result to Output's Unit
46000 Mole per Cubic Meter -->46 Mole per Liter (Check conversion ​here)
FINAL ANSWER
46 Mole per Liter <-- Enzyme Substrate Complex Concentration
(Calculation completed in 00.004 seconds)

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K J Somaiya College of science (K J Somaiya), Mumbai
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Enzyme Conservation Law Calculators

Concentration of Enzyme Catalyst in Presence of Inhibitor by Enzyme Conservation Law
​ LaTeX ​ Go Catalyst Concentration = (Initial Enzyme Concentration-Enzyme Substrate Complex Concentration-Enzyme Inhibitor Complex Concentration)
Concentration of Enzyme Substrate Complex from Enzyme Conservation Law
​ LaTeX ​ Go Enzyme Substrate Complex Concentration = Initial Enzyme Concentration-Catalyst Concentration
Initial Concentration of Enzyme from Enzyme Conservation Law
​ LaTeX ​ Go Initial Enzyme Concentration = Catalyst Concentration+Enzyme Substrate Complex Concentration
Concentration of Enzyme Catalyst by Enzyme Conservation Law
​ LaTeX ​ Go Catalyst Concentration = Initial Enzyme Concentration-Enzyme Substrate Complex Concentration

Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law Formula

​LaTeX ​Go
Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration-Catalyst Concentration-Enzyme Inhibitor Complex Concentration)
ES = ([E0]-E-EI)

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law?

Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law calculator uses Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration-Catalyst Concentration-Enzyme Inhibitor Complex Concentration) to calculate the Enzyme Substrate Complex Concentration, The Concentration of enzyme substrate complex in presence of inhibitor by enzyme conservation law formula is defined as the relation with the concentration of each of the enzyme species of the system. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law using this online calculator? To use this online calculator for Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law, enter Initial Enzyme Concentration ([E0]), Catalyst Concentration (E) & Enzyme Inhibitor Complex Concentration (EI) and hit the calculate button. Here is how the Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law calculation can be explained with given input values -> 0.046 = (100000-25000-29000).

FAQ

What is Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law?
The Concentration of enzyme substrate complex in presence of inhibitor by enzyme conservation law formula is defined as the relation with the concentration of each of the enzyme species of the system and is represented as ES = ([E0]-E-EI) or Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration-Catalyst Concentration-Enzyme Inhibitor Complex Concentration). The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution & The Enzyme Inhibitor Complex Concentration is the number of moles of enzyme inhibitor substrate complex per liter of the enzymatic solution.
How to calculate Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law?
The Concentration of enzyme substrate complex in presence of inhibitor by enzyme conservation law formula is defined as the relation with the concentration of each of the enzyme species of the system is calculated using Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration-Catalyst Concentration-Enzyme Inhibitor Complex Concentration). To calculate Concentration of Enzyme Substrate Complex in presence of Inhibitor by Enzyme Conservation Law, you need Initial Enzyme Concentration ([E0]), Catalyst Concentration (E) & Enzyme Inhibitor Complex Concentration (EI). With our tool, you need to enter the respective value for Initial Enzyme Concentration, Catalyst Concentration & Enzyme Inhibitor Complex Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Enzyme Substrate Complex Concentration?
In this formula, Enzyme Substrate Complex Concentration uses Initial Enzyme Concentration, Catalyst Concentration & Enzyme Inhibitor Complex Concentration. We can use 1 other way(s) to calculate the same, which is/are as follows -
  • Enzyme Substrate Complex Concentration = Initial Enzyme Concentration-Catalyst Concentration
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