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Absorbance using Beer-Lambert Law Calculator

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Beer Lambert law Stark Einstein law Stefan–Boltzmann Law

✖The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength.ⓘ Molar Extinction Coefficient [ε]			+10% -10%
✖The Concentration of Solution is the quantity of a solute that is contained in a particular quantity of solvent or solution.ⓘ Concentration of Solution [c]			+10% -10%
✖Thickness of Cell is useful in calculating the concentration of a solution on the basis of its light absorption.ⓘ Thickness of Cell [l]			+10% -10%

✖Absorbance is also known as optical density of the solution used in calculating the concentration of a solution on the basis of its light absorption.ⓘ Absorbance using Beer-Lambert Law [A]

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Formula

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Absorbance using Beer-Lambert Law

Formula

A = ε \cdot c \cdot l

Example

9.3E-9 = 19 cm²/mol \cdot 97 mol/m³ \cdot 50.5 nm

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Absorbance using Beer-Lambert Law Solution

STEP 0: Pre-Calculation Summary

Formula Used

Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell
A = ε*c*l
This formula uses 4 Variables

Variables Used

Absorbance - Absorbance is also known as optical density of the solution used in calculating the concentration of a solution on the basis of its light absorption.
Molar Extinction Coefficient - (Measured in Square Meter per Mole) - The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength.
Concentration of Solution - (Measured in Mole per Cubic Meter) - The Concentration of Solution is the quantity of a solute that is contained in a particular quantity of solvent or solution.
Thickness of Cell - (Measured in Meter) - Thickness of Cell is useful in calculating the concentration of a solution on the basis of its light absorption.

STEP 1: Convert Input(s) to Base Unit

Molar Extinction Coefficient: 19 Square Centimeter per Mole --> 0.0019 Square Meter per Mole (Check conversion here)
Concentration of Solution: 97 Mole per Cubic Meter --> 97 Mole per Cubic Meter No Conversion Required
Thickness of Cell: 50.5 Nanometer --> 5.05E-08 Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

A = ε*c*l --> 0.0019*97*5.05E-08

Evaluating ... ...

A = 9.30715E-09

STEP 3: Convert Result to Output's Unit

9.30715E-09 --> No Conversion Required

FINAL ANSWER

9.30715E-09 ≈ 9.3E-9 <-- Absorbance

(Calculation completed in 00.004 seconds)

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Created by Akshada Kulkarni

National Institute of Information Technology (NIIT), Neemrana

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< Beer Lambert law Calculators

Concentration of Solution

Go Concentration of Solution = Absorbance/(Thickness of Cell*Molar Extinction Coefficient)

Beer-Lambert law given Intensity of Radiation

Go Absorbance = log10(Intensity of Incident Radiation/Intensity of Transmitted Radiation)

Absorbance using Beer-Lambert Law

Go Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell

Intensity of Incident Radiation

Go Intensity of Incident Radiation = Intensity of Transmitted Radiation*10^(Absorbance)

Absorbance using Beer-Lambert Law Formula

Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell
A = ε*c*l

What is Beer- Lambert Law?

Beer-Lambert law is useful in calculating the concentration of a solution on the basis of its light absorption. This law relates the intensity of the transmitted monochromatic light to the concentration of the solution and the thickness of the cell in which the solution is kept.
The molar extinction coefficient of a substance can be determined using a colorimeter or a spectrophotometer as follows. The absorbances of a solution are measured at different
known concentrations using a cell of known thickness (l). The plot of absorbance, A against Concentration of solution, c gives a straight line and its slope is equal to εl.

Define Photochemistry.

In photochemistry, we study the absorption and emission of light by matter. It consists of the study of various photo physical processes and photochemical reactions. Two important photo physical processes are fluorescence and phosphorescence. During fluorescence, light emission takes place in the presence of exciting radiation; but the light emission stops, once the exciting radiation is removed. In contrast to this, during phosphorescence, light emission takes place even after the removal of the exciting radiation.
In photochemical reactions, the substances acquire the necessary activation energy through light absorption. Again this is in contrast to the thermal reactions in which the reactants acquire their activation energy through collisions between molecules.

How to Calculate Absorbance using Beer-Lambert Law?

Absorbance using Beer-Lambert Law calculator uses Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell to calculate the Absorbance, The Absorbance using Beer-Lambert Law, also known as optical density (OD), is the quantity of light absorbed by a solution. Absorbance is denoted by A symbol.

How to calculate Absorbance using Beer-Lambert Law using this online calculator? To use this online calculator for Absorbance using Beer-Lambert Law, enter Molar Extinction Coefficient (ε), Concentration of Solution (c) & Thickness of Cell (l) and hit the calculate button. Here is how the Absorbance using Beer-Lambert Law calculation can be explained with given input values -> 9.3E-9 = 0.0019*97*5.05E-08.

FAQ

What is Absorbance using Beer-Lambert Law?

The Absorbance using Beer-Lambert Law, also known as optical density (OD), is the quantity of light absorbed by a solution and is represented as A = ε*c*l or Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell. The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength, The Concentration of Solution is the quantity of a solute that is contained in a particular quantity of solvent or solution & Thickness of Cell is useful in calculating the concentration of a solution on the basis of its light absorption.

How to calculate Absorbance using Beer-Lambert Law?

The Absorbance using Beer-Lambert Law, also known as optical density (OD), is the quantity of light absorbed by a solution is calculated using Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell. To calculate Absorbance using Beer-Lambert Law, you need Molar Extinction Coefficient (ε), Concentration of Solution (c) & Thickness of Cell (l). With our tool, you need to enter the respective value for Molar Extinction Coefficient, Concentration of Solution & Thickness of Cell and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Absorbance?

In this formula, Absorbance uses Molar Extinction Coefficient, Concentration of Solution & Thickness of Cell. We can use 1 other way(s) to calculate the same, which is/are as follows -

Absorbance = log10(Intensity of Incident Radiation/Intensity of Transmitted Radiation)

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