Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition Calculator

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%

✖The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.ⓘ Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition [Km^app]

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Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition Solution

STEP 0: Pre-Calculation Summary

Formula Used

Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate
Km^app = (S*(V_max-V₀))/V₀
This formula uses 4 Variables

Variables Used

Apparent Michaelis Constant - (Measured in Mole per Cubic Meter) - The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

STEP 1: Convert Input(s) to Base Unit

Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

Km^app = (S*(V_max-V₀))/V₀ --> (1500*(40000-450))/450

Evaluating ... ...

Km^app = 131833.333333333

STEP 3: Convert Result to Output's Unit

131833.333333333 Mole per Cubic Meter -->131.833333333333 Mole per Liter (Check conversion here)

FINAL ANSWER

131.833333333333 ≈ 131.8333 Mole per Liter <-- Apparent Michaelis Constant

(Calculation completed in 00.007 seconds)

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< Competitive Inhibitor Calculators

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

LaTeX Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

LaTeX Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

LaTeX Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition Formula

LaTeX Go

Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate
Km^app = (S*(V_max-V₀))/V₀

What is apparent michaelis constant?

Apparent Km is the Michaelis constant as observed under conditions (e.g. the presence of a competitive inhibitor) that would hinder the determination of its true value; in the case of a two-substrate enzyme, the Michaelis constant measured under the particular conditions of a defined concentration of the invariant substrate.

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition?

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition calculator uses Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate to calculate the Apparent Michaelis Constant, The Apparent value of Michaelis Menten constant in presence of Competitive inhibition formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km(app). Apparent Michaelis Constant is denoted by Km^app symbol.

How to calculate Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition using this online calculator? To use this online calculator for Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition, enter Substrate Concentration (S), Maximum Rate (V_max) & Initial Reaction Rate (V₀) and hit the calculate button. Here is how the Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition calculation can be explained with given input values -> 0.131833 = (1500*(40000-450))/450.

FAQ

What is Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition?

The Apparent value of Michaelis Menten constant in presence of Competitive inhibition formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km(app) and is represented as Km^app = (S*(V_max-V₀))/V₀ or Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate. The Substrate Concentration is the number of moles of substrate per liter solution, The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration & The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

How to calculate Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition?

The Apparent value of Michaelis Menten constant in presence of Competitive inhibition formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km(app) is calculated using Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate. To calculate Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition, you need Substrate Concentration (S), Maximum Rate (V_max) & Initial Reaction Rate (V₀). With our tool, you need to enter the respective value for Substrate Concentration, Maximum Rate & Initial Reaction Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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